Effects of hepatitis B virus on lipid metabolism related proteins in human hepatoma cell lines
YAN Cai-ling, PENG Xian-e, WU Yun-li, LIN Xu, CHEN Wan-nan
Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Key Laboratory of Fujian Provincefor Tumor Microbiology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350108, China
Abstract：In this study, we aimed to investigate the effects of hepatitis B virus on lipid metabolism related proteins both in mRNA and protein levels. To address these issues, six proteins, including apolipoprotein A-I (ApoAI), apolipoprotein E (ApoE), superoxide dismutase 2 (SOD2), NAD(P)H dehydrogenase, quinone 1 (NQO1), fatty acid binding protein 1 (FABP1) and acetyl-Coenzyme A acetyltransferase 2(ACAT2) in hepatoma cells either harboring HBV or transiently transfected with 1.2×length of HBV genome, were detected by real-time PCR or western blot analysis. Results revealed that HBV could down-regulate the expression of SOD2 and NQO1, while up-regulate the expression of FABP1, which suggested that HBV may interfere with the expression of lipid metabolism proteins, and attribute to hepatocellular steatosis.
颜彩玲, 彭仙娥, 吴云丽, 林旭, 陈婉南. 乙型肝炎病毒对人肝癌细胞脂类代谢相关蛋白表达的影响[J]. 中国人兽共患病学报, 2015, 31(6): 560-564.
YAN Cai-ling, PENG Xian-e, WU Yun-li, LIN Xu, CHEN Wan-nan. Effects of hepatitis B virus on lipid metabolism related proteins in human hepatoma cell lines. Chinese Journal of Zoonoses, 2015, 31(6): 560-564.
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2015, Vol. 31 
