Abstract:Liposome-encapsulated dichloromethylene diphosphonate (LE-Cl2MDP) was prepared to selectively eliminate out Kupffer cells in a mouse model of Schistosoma japonica infection. Further studies by inhibiting the effects of Kupffer cell function were implemented to judge whether it impacted on the liver granuloma formation of Schistosoma japonica infection. Liposome was used to package CL2MDP. The 6-8 weeks old female BALB/c mice were divided into three groups, including phosphate buffered saline (PBS) group, Schistosoma japonica infection group and Schistosoma japonica infection combined LE-Cl2BMP group. The combination group model was reproduced by injection of LE-Cl2BMP into tail vein (0.1 mL/10 g) for twice a week through six weeks. Mice were sacrificed at 12 week post infection with Schistosoma japonicum, serum and liver specimens were collected to detect cytokines and immunohistochemistry, etc. Statistical analysis was performed based on relevant data. Results showed that after LE-Cl2MDP treatment, Kupffer cells of Schistosoma japonica egg granuloma significantly reduced. Inflammatory cells infiltration surrounding schistosome egg granuloma in liver also decreased significantly, and that alleviated hepatic fibrosis at 8 or 12 week. At 12 weeks after infection, the level of IL-10 in the sera of the mice with schistosomiasis infection combined LE-Cl2MDP group (42.6 10.4) pg/mL was significantly lower than that of schistosomiasis infection group (67.4 12.9) (P<0.05). This study established the model that LE-Cl2MDP was able to eliminate the hepatic Kupffer cells in the mice infected with Schistosoma japonicum, and it also inhibited inflammatory response around schistosomal egg granuloma.
杨江华, 孙静, 梁曼曼, 盛浩宇, 王文节. 抑制Kupffer细胞功能减轻日本血吸虫病肝脏肉芽肿炎症反应[J]. 中国人兽共患病学报, 2015, 31(7): 623-626.
YANG Jiang-hua, SUN Jing, LIANG Man-man, SHEN Hao-yu, WANG Wen-jie. Inflammatory response of schistosomiasis hepatic granuloma by inhibiting the function of Kupffer cells. Chinese Journal of Zoonoses, 2015, 31(7): 623-626.
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