Abstract：We investigated the rodent carrying the Hantaan virus (HV) and the characteristics of molecular epidemiology at Heilongjiang frontier ports at Sino-Russia border. The night trapping and mouse cages were used to capture rodents, rodent lung samples were detected by nested PCR method, positive samples were sequenced and genotyped, and obtained sequences were then analyzed by MEGA6. In this survey, we captured 346 rodents, identified to be 3 families, 9 genera and 11 species. A total of 17 samples carried Hantavirus were with the infection rate of 4.9%. In which, 6 samples belonged to HNTV and 11 samples to SEOV, which was the first case of SEOV detected from Microtus fortis in Heilongjiang Port, China. Phylogenetic analysis showed that 6 strains of Hantavirus were not closely related to the prototype strain Hantaan76-118 (homology was 86.82%-88.43%), but showed high homology with HNT BAO14 and HNT HXZ244 strains. The 11 strains of Seoul virus showed high homology with SEO Gongzhuling97, SEO BjHD01, and SEO HaiPhong3-11 (isolated from Vietnam) strains respectively. Based on the above results, we can analyse that at least two types of Hantavirus infected by rodent at Heilongjiang Sino-Russian border, and a relatively high infection rate suggested the importance of increasing monitoring and control HFRS at Sino-Russian border areas.
程成, 鞠文东, 付维明, 曹苏娅, 徐宁, 王艳梅, 耿聪, 王玉龙. 黑龙江口岸地区鼠类动物中汉坦病毒感染情况调查[J]. 中国人兽共患病学报, 2015, 31(7): 681-686.
CHENG Cheng, JU Wen-dong, FU Wei-ming, CAO Su-ya, XU Ning, WANG Yan-mei, GENG Cong, WANG Yu-long. Epidemiologic investigation on Hantavirus carried by rodents at Heilongjiang frontier ports. Chinese Journal of Zoonoses, 2015, 31(7): 681-686.
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2015, Vol. 31 
