Enhancement of epitope vaccines from wx2b4a of Toxoplasma gondii potency using Astragalan and Lentinan in mice
XIE Rong-hua1, FAN Jiu-bo2, SHU Heng-ping3
1.Environment Biology of Profession Technology College in Hunan, Hengyang 421001, China; 2.Xiangfan Central Hospital, Xiangfan 441021, China; 3.Department of Parositology, Xiangya School of Medicine, Central South University, Changsha 410078, China
Abstract：We observed the effect of Astragalan and Lentinan to enhance the immune response and protective immunity induced by the epitope vaccines from wx2b4a of Toxoplasma gondii in mice. Mice were immunized intramuscularly with Astragalan and pcDNA3-W2b4a, Lentinan and pcDNA3-W2b4a, respectively. The level of IgG antibodies of mice were detected by ELISA. In vitro, the levels of IL-2 and IFN-γ that were secreted from pcDNA3-W2b4a-stimulated splenocytes were also measured by ELISA. Proliferation of T cells was detected by CCK-8 and mouse survival challenged by T. gondii was observed. Results showed that after the immunization, the level of IgG antibodies in immunized groups were significantly higher than those in the control group (P<0.05). The level of IgG antibodies in sera of mice inoculated with Astragalan and pcDNA3-W2b4a were higher than that in Lentinan and pcDNA3-W2b4a (P<0.05). After the immunization, the level of IL-2 and IFN-γ in splenoctes of mice inoculated with Astragalan and pcDNA3-W2b4a, Lentinan and pcDNA3-W2b4a were significantly higher than that those in GrouppcDNA3-W2b4a (all P<0.05). After the immunization, proliferation of T cells in immunized groups were significantly higher than those in the control group (P<0.05). The proliferation of T cells inoculated with Lentinan and pcDNA3-W2b4a were higher than Astragalan and pcDNA3-W2b4a (P<0.05). Following challenging with RH tachyzoites, the mean survival time in Astragalan and pcDNA3-W2b4a group, Lentinan and pcDNA3-W2b4a group were significantly longer than those in Group pcDNA3-W2b4a (all P<0.05). It indicated that Astragalan and Lentinan could enhance wx2b4a immune response that exerts a superior immune protection, which might be a new practical strategy for developing Toxoplasma gondii vaccines.
谢荣华, 范久波, 舒衡平. 黄芪多糖、香菇多糖增强弓形虫wx2b4a表位疫苗免疫小鼠的保护作用[J]. 中国人兽共患病学报, 2015, 31(8): 724-727.
XIE Rong-hua, FAN Jiu-bo, SHU Heng-ping. Enhancement of epitope vaccines from wx2b4a of Toxoplasma gondii potency using Astragalan and Lentinan in mice. Chinese Journal of Zoonoses, 2015, 31(8): 724-727.
[1] Fan JB, Wu X, Tan K, et al. The construction and characterization of multiple-epitopes vaccine of new genes of Toxoplasma gondii [J]. J Pathog Biol, 2007, 2(2): 118-121. (in Chinese) 范久波,吴翔,谭逵,等.弓形虫新基因表位疫苗质粒的构建及鉴定[J].中国病原生物学杂志,2007,2(2):118-121. [2] Fan JB, Wu X, Tan K, et al. The protective immunity induced by the epitope vaccines from two new novel genes of Toxoplasma gondii in mice[J]. J Pathog Biol, 2008, 3(6): 421-424. (in Chinese) 范久波,吴翔,谭逵,等.弓形虫新基因表位疫苗效果的观察[J].中国病原生物学杂志, 2008, 3(6): 421-424. [3] He YS. Study on the immune adjusting reagent of chinese medicine and its anti-tumor effect[J]. Occupat Health, 2005, 21(10):1454-1459. (in Chinese) 何跃生.中草药免疫调节剂及抗肿瘤作用研究进展[J].职业与健康,2005,21(10):1454-1459. [4] Zhao D, Chang J. Advances in the studies on the mechanism of immuno-potentiation effect of chinese material medica[J]. J Baotou Med Coll, 2013, 29(4): 122-124. (in Chinese) 赵东,常江.中药有效成分免疫增强作用机制的研究概况[J]. 包头医学院学报,2013,29(4):122-124. [5] Dai QM, Liu L, Cai LS, et al. Regulatory effect of Lentinan on CD4 + CD25 + Foxp3 + regulatory T cells in BALB/ c mice infected with Toxoplasma gondii [J]. Chin J Microecol, 2013,25(5): 524-527.(in Chinese) 代巧妹,刘蕾,蔡连顺,等.香菇多糖对急性弓形虫感染过程中CD4 + CD25 + Foxp3 + 调节性T 细胞的调节作用研究[J].中国微生态学杂志,2013,25(5):524-527. [6] Wang GX, Lin WM, Zhao RF, et al. Effects of six polysaccharides extracted from plants on the immunological cells of mice[J]. J Hyg Res, 2008, 37(5): 577-580. (in Chinese) 王国秀,蔺威鸣,赵瑞芳,等.6 种多糖对小鼠免疫细胞活性作用的比较研究[J]. 卫生研究,2008,37(5):577-580. [7] Huang DS, Guo AZ. Effects of immunity in Astragalan adjuvan on swine plague vaccine on piglets[J]. China Anim Husb Vet Med, 2011, 38(6): 189-192. (in Chinese) 黄德尚,郭爱珍.黄芪多糖佐剂对猪瘟疫苗免疫效果的影响[J].中国畜牧兽医,2011,38(6):189-192. [8] Guo P, Li CL, Huang KS, et al. Promotion effects of Lentinan on immunologic function of BALB/c Murine peritoneal macrophage against Toxoplasma Gondii infection[J]. China Pharm, 2011, 22(3): 215-217. (in Chinese) 葛璞,李春莉,黄开顺,等.香菇多糖促BALB/c小鼠腹腔巨噬细胞抗弓形虫感染的研究[J].中国药房,2011,22(3):215-217. [9] Wang TX, Wang TX, Wu GC, et al. Effect of Astragalus polysaccharideon immunological function in mice[J]. Lishizhen Med Materia Medica Res, 2009,20(7): 1763-1764.(in Chinese) 王庭欣,王庭祥,吴广臣,等.黄芪多糖增强小鼠免疫功能的实验研究[J].时珍国医国药,2009,20(7):1763-1764.
2015, Vol. 31 
