Abstract:We conducted evaluation on the performance of GenoType MTBDRplus assay for rapid detecting multi-drug resistant Mycobacterium tuberculosis Complex (MDR-MTBC) isolates in Fujian Province, China. The strains including 79 MDR and 50 pan-susceptible were chosen randomly from 30 survey sites. The MTBDRplus was applied to detect RFP and INH resistance of the strains, and the results were compared with the result of sequencing data about rpoB, katG and inhA mutation. The Tub control were positive in all strains (100%), the results showed the patterns of 50 pan-susceptible strains were concordant with DST. By the assay, 67 (84.81%) MDR strains of 79 were correctly identified, 10 strains (12.66%) were mono resistant to RFP, and 2 were mono resistant to INH. The mutation in rpoB gene of 77 MDR strains and in katG / inhA gene of 71 MDR strains was detected by sequencing. Provided that conventional DST is the "gold standard," the sensitivity of the MTBDR assay were 97.47% for RFP resistance detection, 87.34% for INH resistance detection and 84.81% for MDR detection. And the assay had the specificity of 100% for the detection of RFP, INH and MDR. The concordance rate of detection for RFP, INH and MDR was 98.45% (127/129), 92.25% (119/129), 90.70% (117/129), respectively. Thus, the new GenoType MTBDRplus assay represents a rapid, reliable and easy tool for the detection of INH and RFP resistance in clinical strains of MDR in Fujian Province and the sensitivity needs to be improved.
魏淑贞, 林淑芳, 林建, 赵永, 梁庆福, 林勇明. GenoType MTBDRplus快速检测耐多药结核分枝杆菌复合群临床分离株效果评价[J]. 中国人兽共患病学报, 2015, 31(8): 728-732.
WEI Shu-zhen, LIN Shu-fang, ZHAO Yong, LIN Jin, LIANG Qing-fu, LIN Yong-ming. Evaluation on the performance of GenoType MTBDRplus assay for rapid diagnosisMDR Mycobacterium tuberculosis isolates in Fujian Province, China. Chinese Journal of Zoonoses, 2015, 31(8): 728-732.
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