Abstract:We studied the immunomodulatory effect of Astragalus on immune mice from the epitope vaccines wx2b2a of Toxoplasma gondii (T. gondii) against acute T. gondii infection. The mice were randomly divided into Group pcDNA3-W2b2a, Group pcDNA3-W2b2a + Astragalus treatment, Group pcDNA3 + Astragalus treatment, Group Astragalus treatment, and Group pcDNA3 and physiological saline. The mice in Group pcDNA3-W2b2a and Group pcDNA3-W2b2a + Astragalus treatment were intramuscularly immunized with pcDNA3-W2b2a of T. gondii for three times. Four weeks after the last immunization, the mice in each group were intraperitoneally injected with 102 tachyzoites. Starting from 2 days after infection, the mice in Group pcDNA3-W2b2a + Astragalus treatment, Group pcDNA3 + Astragalus treatment, Group Astragalus treatment were orally treated with Astragalus with 75 mg/d for 7 days. ELISA method was taken to assay the serum levels of IgG before immunization, 4 weeks after the last immunization and 6 day post-infection (dpi) , and the levels of IFN-γ and IL-18 before immunization, 2 weeks after the last immunization and 4, 6 and 8 dpi, as well as to observe the survival time of the mice attacked by T. gondii. The serum levels of IgG of the mice which were immune with pcDNA3-W2b2a were higher than those in other groups (P<0.05). The levels of IgG antibodies in Group pcDNA3-W2b2a, Group pcDNA3-W2b2a + Astragalus treatment were almost the same in four weeks after the last immunization and 6 dpi (P>0.05). The levels of IFN-γ in Group pcDNA3-W2b2a + Astragalus treatment were higher than those in other groups (P<0.05) in 2 weeks after the last immunization and 4, 6, 8 dpi. The serum levels of IL-18 kept elevating after infection. But they are significantly lower than those in Group pcDNA3 and physiological saline after 8 days with Astragalus treatment (P<0.05). After infection with RH tachyzoites, the mice in Group pcDNA3-W2b2a + Astragalus treatment lived longer than those in Group pcDNA3-W2b2a, Group pcDNA3 + Astragalus treatment, and Group Astragalus treatment (P<0.05). Astragalus can strengthen the immunomodulatory effect on immune mice from the epitope vaccines wx2b2a of T. gondii against acute T. gondii infection.
谢荣华, 舒衡平. 黄芪对弓形虫wx2b2a表位疫苗免疫小鼠后抗急性弓形虫感染的免疫调节作用[J]. 中国人兽共患病学报, 2015, 31(9): 822-825.
XIE Rong-hua, SHU Heng-ping. Immunomodulatory effect of Astragalus on immune mice from the epitope vaccines wx2b2a of Toxoplasma gondii against acute T. gondii infection. Chinese Journal of Zoonoses, 2015, 31(9): 822-825.
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