A new method based on the multiple locus variable number tandem repeat analysis (MLVA) was applied for the genotyping of combined HIV Mycobacterium tuberculosis in Dali to investigate the genotyping and distribution pattern of Mycobacterium tuberculosis clinical isolates with MLVA. Mycobacterium tuberculosis clinical isolates were selected from Dali area, and the polymorphism of VNTR locus was tested with PCR. The clustering of genotype was analyzed by BioNumerics(6.6). Result showed that 15 VNTR loci of 61 combined HIV Mycobacterium tuberculosis clinical isolates were analyzed respectively. There were obvious polymorphisms of VNTRs. The discrimination power of these loci appeared different from each other, with the biggest Hunter-Gaston index (0.839) loci was MIRU26, and the smallest one (0.341) loci was MIRU4. The clustering of genotype showed that these strains could be categorized into 5 gene clusters and 61 genotype, the proportions of cluster Ⅰ was the biggest one, 51.6% were cluster Ⅰ which including 32 strains. The standard strain H37Rv was belongs to cluster Ⅱ. Its indicated that there are obvious polymorphisms of VNTRs of combined HIV Mycobacterium tuberculosis clinical isolates in Dali. The main genotype was Beijing family genotype.
聂恒, 王旭东, 吴利先. 大理地区合并HIV双重感染者结核分枝杆菌MLVA法基因分型研究[J]. 中国人兽共患病学报, 2015, 31(10): 923-926.
NIE Heng, WANG Xu-dong, WU Li-xian. MLVA based Mycobacterium tuberculosis clinical isolates genotyping analysis from HIV co-infection patients in Dali area, China. Chinese Journal of Zoonoses, 2015, 31(10): 923-926.
[1] Zhang K, Ma DQ, Lv FJ, et al. Clinical diagnosis and treatment of acquired immunodeficiency syndrome complicated by tuberculosis[J]. Chin J Tuberc Respir Dis, 2001, 24(11): 682-684. (in Chinese) 张可,马大庆,吕富靖,等.艾滋病合并结核病的诊断与治疗[J].中华结核和呼吸杂志,2001,24(11):682-684.
[2] Zhao YD, Feng Q, Sun Q, et al. Application of variable number tandem repeats in genotyping of Mycobacterium tuberculosis strains in Sichuan[J]. J Sichuan Univ (Nat Sci Ed), 2012, 49(4): 909-913. (in Chinese) 赵于丁,冯钦,孙群,等. 应用多位点数目可变串联重复序列对四川地区结核分枝杆菌的基因分型[J].四川大学学报:自然科学版,2012,49(4):909-913.
[3] Supply P, Allix C, Lesjean S, et al. Proposal for standardization of optimized mycobacterial interspersed repetitive unit variable-number tandem repeat typing of Mycobacterium tuberculosis [J]. J Clin Microbiol, 2006, 44(12): 4498-4510. doi:10.1128/JCM.01392-06
[4] Liang QF, Chen QY, Zheng JF, et al. Application of variable number tandem repeats in genotyping of Mycobacterium tuberculosis strains in Fujian[J]. Chin J Epidemiol, 2012, 33(11): 1167-1170. (in Chinese) 梁庆福,陈求扬,郑金凤,等. 福建省结核分枝杆菌多位点可变串联重复序列基因分型研究[J].中华流行病学杂志,2012,33(11):1167-1170.
[5] Kam KM, Yip CW, Tse LW, et al. Utility of mycobacterial interspersed repetitive unit typing for differentiating multidrug-resistant Mycobacterium tuberculosis isolates of the Beijing family[J]. J Clin Microbiol, 2005, 43(1): 306-313. doi:10.1128/JCM.43.1.306-313.2005
[6] Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity[J]. J Clin Microbiol, 1988, 26(11): 2465-2466.
[7] Supply P, Mazars E, Lesjean S, et al. Variable human minisatellite-like regions in the Mycobacterium tuberculosis genome[J]. Mol Microbiol, 2000, 36(3): 762-771. doi:10.1046/j.1365-2958.2000.01905.x
[8] Crawford JT. Genotyping in contact investigations: a CDC perspective[J]. Int J Tuberc Lung Dis, 2003, 7(12 Suppl 3): S453-457.
[9] Zheng XZ, Zheng SH, Du BP, et al. The application of multiple loci variable number tandem repeats analysis in genotyping of Beijing family Mycobacterium tuberculosis strains[J]. J Clin Pulmon Med, 2010, 15(7): 957-961. (in Chinese) 郑晓静, 郑素华, 杜博平,等.多位点可变数量串联重复序列分析在北京家族结核分枝杆菌基因分型中的应用及位点筛选[J].临床肺科杂志,2010,15(7): 957-961.
[10] Liu RX, Xing LL, Peng Z, et al. Usefulness of mycobacterial interspersed repetitive-unit locus PCR amplification in rapid diagnosis of Beijing lineage strain infection among pediatric tuberculosis patients[J]. J Clin Microbiol, 2011, 49(2): 712-714. doi:10.1128/JCM.01694-10
[11] Luo T, Yang C, Gao Q. Mycobacterial interspersed repetitive-unit locus PCR amplification and Beijing strains of Mycobacterium tuberculosis [J]. J Clin Microbiol, 2011, 49(11): 4026-4027. doi:10.1128/JCM.05462-11
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