In this study, the objective is to establish a nested-PCR assay for the detection of H. bilis with high sensitivity and specificity. The nested primers were designed based on sequences of 16S rRNA gene of seventeen subtypes of H. bilis. After optimizing reaction condition, the sensitivity and specificity of the assay were examined via the detection of feces simulated samples, mice infection model samples and clinic patients’ samples. The detection sensitivity of H. bilis strain for feces simulated samples was 10 CFU/100 μL. H. bilis was successfully detected in the liver, caecum and feces of experimentally infected mice. Moreover, H. bilis was successfully detected in the bile, cholecyst mucous membrane and feces samples from two of ten patients with cholelithiasis. Due to the PCR assay’s high sensitivity and specificity, the method may be used to detect the infection of H. bilis.
秦和平, 孙勇, 叶安丽, 潘信义. 一种检测胆螺杆菌套式PCR方法的建立[J]. 中国人兽共患病学报, 2015, 31(10): 943-946.
QIN He-ping, SUN Yong, YE An-li, PAN Xin-yi. Development of a nested PCR assay for detection of Helicobacter bilis. Chinese Journal of Zoonoses, 2015, 31(10): 943-946.
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