We investigated the distribution of Chlamydia trachomatis genotypes in the outpatients visiting STD clinics in Fuzhou City, China. Between 2013 and 2014, a total of 2 019 urethral or endocervical specimens from susceptible genital C. trachomatis infections were collected and detected by using real-time PCR assay kit. The C. trachomatis -positive specimens were subsequently amplified with nested PCR for Omp1 region and analyzed by sequencing. Genotyping was performed by BLAST similarity search and phylogenetic tree analysis. Eighty-three of the 86 C. trachomatis-positive specimens were successfully sequenced. Only one specimen was identified as multiple infection. Eight different genotypes were identified. The most prevalent was F (36.59%), followed by E (24.39%), D (12.20%), G (9.76%), J (9.76%), H (3.66%), K (2.44%) and B (1.22%). Synonymous mutation rate and non-synonymous mutation rate among Omp1 genes of different genotypes were calculated. All genotypes but G showed dN/dS values of<1. Genotypes F and E were commonest. Synonymous substitution was the major pattern of variation in the process of Omp1 gene evolution.
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