Effect of cell activity of protoscolices by super absorbent resin in coordination with high intensity focused ultrasound
ZHANG Jing1, 2, WANG Meng-ying2, YE Bin1, 2, ZHAO Yi-feng3, HAN Xiu-ming4, LI Fa-qi5, WANG Qi5
1.Department of Pathogenic Biology, Chongqing Medical University, Chongqing 400016, China; 2.Research Center for Molecular Medicine and Tumor, Chongqing Medical University, Chongqing 400016, China; 3.Department of Oncology, the Affiliated Hospital of Qinghai University, Qinghai 810001, China; 4.Qinghai Institute for Endemic Disease Prevention and Control, Qinghai 811602, China; 5.State Key Laboratory of Ultrasound Engineering in Medicine Co-founded by Chongqing and the Ministry of Science and Technology, Chongqing 400016, China
Abstract:We investigated the effects of a combination of high intensity focused ultrasound (HIFU) and super absorbent resin on the cell activities of Echinococcus granulosus protoscolices with enzyme histochemical technique in vitro. The suspension with 2 000 protoscoleces/ML from the hydatid cysts in sample sheep liver was divided into groups: group I (blank control) was without HIFU treatment and SAR in the suspension, group II was only SAR, group III was treated with HIFU only, group IV was treated with HIFU and 0.01 g SAR, and groupⅤ was treated with HIFU and 0.1g SAR. Then, all these groups were exposed to the suspension of HIFU at a dose of 100 W acoustic powers for 30 seconds. The HIFU treated protoscolices were smeared onto glass slides for enzymatic activities localization. The protoscolex morphological changes and related enzyme activities in the HIFU treated protoscolices were determinated by the histochemical staining methods. Results showed the morphological changes of protoscoleces, the effect relationship was with dose of SAR, SAR was larger and the extent of damage was higher. There were clear and intact form in the nomal group, however, the protoscoleces showed stained and disintegrated in the tested groups. The death rate in the duratioh of single HIFU group was 80.1%, they were 87.82% and 89.1% respectively in the HIFU combined SAR groups. In the groups of the combination of HIFU and SAR, the main enzyme color of protoscoleces in shallow or disappeared, which suggested the treatment had an influence on enzyme activities of protoscolices. In conclusion, SAR could increase HIFU irradiation on the acute cytotoxicity and has certain delayed effect on protoscoleces, and the effect of HIFU on G-6-P and SDH in E. granulosus protoscolices was significantly low.
张静, 王梦莹, 叶彬, 赵毅峰, 韩秀敏, 李发琪, 王琦. 高吸水性树脂协同高强度聚焦超声对细粒棘球绦蚴原头节细胞酶活性的影响[J]. 中国人兽共患病学报, 2015, 31(11): 987-993.
ZHANG Jing, WANG Meng-ying, YE Bin, ZHAO Yi-feng, HAN Xiu-ming, LI Fa-qi, WANG Qi. Effect of cell activity of protoscolices by super absorbent resin in coordination with high intensity focused ultrasound. Chinese Journal of Zoonoses, 2015, 31(11): 987-993.
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