Species and subtype identification of Cryptosporidium spp.from dairy cattle in an imported farm and its biosafety evaluation
WANG Chen-rong, QI Meng, LI Jun-qiang, ZHANG Zhen-jie, LIU Peng-fei, YU Fu-chang, CAO Jian-ke, NING Chang-shen, ZHANG Long-xian
College of Animal Science and Veterinary Medicine, Henan Agricultural University / International Joint Research Laboratory for Zoonotic Diseases of Henan Province, Zhengzhou 450002, China
Abstract：We investigated the effect of Cryptosporidium from imported cow in the local area and the distribution effect of Cryptosporidium species and genotypes in the local area. Using polymerase chain reaction (PCR), we studied the infection of Cryptosporidium from dairy cattle in an imported farm in Henan Province. The overall infection rate of Cryptosporidium in dairy cattle was 17.8% (90/507) based on 18SRNA gene locus. Cryptosporidium parvum, Cryptosporidium bovis, Cryptosporidium ryanae and Cryptosporidium andersoni were found. Significant differences were observed between the infection rate of sampling and age (P<0.01). The infection rate of Cryptosporidium decreased with age increasing in dairy cattle. Cryptosporidium parvum were the dominating Cryptosporidium spp. in pre-weaning calves. We found a positively correlation between the infection of Cryptosporidium and diarrhea of pre-weaning calves (P<0.01). Thirty-five Cryptosporidium parvum were successfully amplified from 43 positive samples at gp60 gene locus, the subtype of the amplified Cryptosporidium parvum were IIdA19G1, the subtype was different with IIa subtype which were found in imported country (Australia). This study confirmed that Cryptosporidium parvum is a kind of diarrhea pathogens in dairy calves, the subtype of Cryptosporidium parvum in China is only IId and the subtype of Cryptosporidium parvum exist geographical isolation genetic characteristics. It does not have the importance of biological safety from the point that imported heifers and adults do not affect the distribution of Cryptosporidium species and genotypes in the local area.
王臣荣， 齐萌， 李俊强， 张振杰， 刘鹏飞， 余复昌， 曹建科， 宁长申， 张龙现. 规模化引种场进口奶牛隐孢子虫种类、基因亚型鉴定及其生物安全评价[J]. 中国人兽共患病学报, 2015, 31(11): 1005-1009.
WANG Chen-rong, QI Meng, LI Jun-qiang, ZHANG Zhen-jie, LIU Peng-fei, YU Fu-chang, CAO Jian-ke, NING Chang-shen, ZHANG Long-xian. Species and subtype identification of Cryptosporidium spp.from dairy cattle in an imported farm and its biosafety evaluation. Chinese Journal of Zoonoses, 2015, 31(11): 1005-1009.
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