Identification and molecular characteristics of important virulence genes of porcine pseudorabies virus strain Jiangxi-FZ
FAN Ke-wei1,2, DAI Ai-ling1,2, WU De-feng3, LIN Wei-ming1,2, LU Ma-ying1, YANG Xiao-yan1,2
1.College of Life Sciences, Longyan University, Longyan 364000, China; 2.Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology, Longyan 364000, China; 3.College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract:To study the molecular characteristics of important virulence genes of Porcine pseudorabies virus (PRV) new epidemic strains, one local strain of PRV was isolated from the brain of a 23-day old pig in Jiangxi Province, and was identified by necropsy, PCR, virus isolation and culture, IFA and animal test. It was named as the PRV Jiangxi-FZ strain. The analysis of molecular characteristic and phylogenetic relationship of its important virulence genes TK, gB, gC, gD and gE showed that the TK, gB, gC, gD and gE gene sequences of Jiangxi-FZ strain shared a strictly conservative with other reference PRV strains at the nucleotide and amino acid levels, especially shared higher homology with 2 PRV variants isolated in 2012. However, there were still some differences between the nucleotide sequences and amino acid sequences of TK, gB, gC, gD and gE genes of Jiangxi-FZ strain and other reference PRV strains on the highly conservative basis, and a part of these differences were characteristic. Phylogenetic analysis showed that the PRV Jiangxi-FZ strain and the PRVs isolated from different provinces in China since 2012 had more closely relationship far away from the European and American PRV strains. Jiangxi-FZ strain had a certain representative of the PRV current epidemic strains, which belong to the PRV variants prevailing in China in recent years.
范克伟, 戴爱玲, 吴德峰, 林炜明, 卢马英, 杨小燕. 猪伪狂犬病毒Jiangxi-FZ株的分离鉴定及其重要毒力基因分子特征[J]. 中国人兽共患病学报, 2015, 31(12): 1103-1110.
FAN Ke-wei, DAI Ai-ling, WU De-feng, LIN Wei-ming, LU Ma-ying, YANG Xiao-yan. Identification and molecular characteristics of important virulence genes of porcine pseudorabies virus strain Jiangxi-FZ. Chinese Journal of Zoonoses, 2015, 31(12): 1103-1110.
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