Spoligotyping of Mycobacterium tuberculosis in the South of Sichuan Province
DENG Jian-ping1, LIU Hai-can2, WANG Bin1, DONG Hai-yan2, ZHANG Zheng-dong1, ZHAO Xiu-qin2, LI Qun1, WAN Kang-lin2
1. Zigong Center for Disease Control and Prevention, Zigong 643000, China; 2.State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：We investigated the distribution of genotypes of Mycobacterium (M.) tuberculosis in the South of Sichuan Province. And furtherly, to provide the basis on molecular epidemiology for tuberculosis (TB) prevention and control. In the year of 2010, 267 clinical isolates were genotyped by using spacer oligonucleotide typing (Spoligotyping) in the South of Sichuan. After that, BioNumerics (Version 5.10) software was used to analysis the results and then compared them to the SpolDB4 database. Of the 267 M. tuberculosis isolates, there were 66 genotypes. Of which 51 strains showed unique genotypes, and the remaining 216 strains were grouped into 10 gene clusters. By comparing with the SpolDB4.0 database,we found that 144 strains belonged to the Beijing family (53.93%), 79 strains were the T family (29.59%), nine H-family strains, three U-family strains, two MANU-family strains, the other 30 strains showed strange genotype that didn't exist in the SpolDB4.0 database. In the South of Sichuan, M. tuberculosis has high gene polymorphism, Beijing family and T family strains are the main epidemic strains. It’s necessary to strengthen the monitoring and biological characteristics research of the two genotypes strains.
邓建平， 刘海灿， 王斌， 董海燕， 张正东， 赵秀芹， 李群， 万康林. 四川省川南地区结核分枝杆菌Spoligotyping分型研究[J]. 中国人兽共患病学报, 2015, 31(12): 1116-1119.
DENG Jian-ping, LIU Hai-can, WANG Bin, DONG Hai-yan, ZHANG Zheng-dong, ZHAO Xiu-qin, LI Qun, WAN Kang-lin. Spoligotyping of Mycobacterium tuberculosis in the South of Sichuan Province. Chinese Journal of Zoonoses, 2015, 31(12): 1116-1119.
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2015, Vol. 31 
