Abstract:In order to study handy and quick diagnosis of candidiasis albicans of broiler chicken, assays using 50 clinical suspected samples from several chicken farms were performed by methods of KOH microscopic examination, modified KOH microscopic examination, morphological observation, TTC chromogenic culture, histologic section technique, and nested PCR technique. The positive rates of detected samples by methods above were 62.0%(31/50), 78.0%(39/50), 82.0%(41/50), 82.0%(41/50), 86%(43/50) and 88.0%(44/50), and the time drains were 1-2 hours(h), 1-2 h, 7-8 d, 6-7 d, 8-10 d and 7-8 h, respectively. By comparison of merits and demerits between different methods, a proposal of combining the nested PCR test with the modified microscopic examination is presented as a preferred embodiment for handy and quick diagnosis of candidiasis albicans in broiler chicken, which has advantages including high specificity and sensitivity, less time consumption and taking account of visualizability of the invasive pathogen.
刘建钗, 柳焕章, 刘彦威, 王慧真, 张永英. 肉鸡白念珠菌病常用实验诊断方法比较研究[J]. 中国人兽共患病学报, 2015, 31(12): 1151-1156.
LIU Jian-chai, LIU Huan-zhang, LIU Yan-wei, WANG Hui-zhen, ZHANG Yong-ying. Comparison research on commonly experimental diagnostic methods of candidiasis albicans in broiler chicken. Chinese Journal of Zoonoses, 2015, 31(12): 1151-1156.
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