Identification and molecular characteristic of Chlamydophila abortus Guangdong strain in goats
WANG Ling-ling, LIU Yong-jie, ZHENG Hai-xue, ZHANG Ke-shan, LIU Xiang-tao
Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou 730046, China
Abstract:To identify suspected goats enzootic abortion cases and analyze the molecular characteristic of the pathogen, tissue samples were collected and detected by pathology examination, GIMSA dying assay, microscopic examination, specific gene amplification and sequencing, and the phylogenic tree based on gene ompA was performed. Results showed that the pathologic changes were similar with Chlamydophila abortus (C. abortus) infection, the typical characters of Chlamydia infection were observed by microscope after GIMSA dying and specific gene ompA was amplified by PCR, indicating the disease was caused by C. abortus. Gene ompA sequence was submitted to GenBank and obtained accession number KP984478. Phylogenetic tree analysis revealed that the C. abortus strain identified had a closest genetic relationship with KF130872 isolated from Guizhou in 2013(with 99.1% homology), and had the farthest relationship with AJ004873 published in 2002(with 98.2% homology). Results of this paper laid the foundation for epidemiologic further study on C. abortus.
王玲玲, 刘永杰, 郑海学, 张克山, 刘湘涛. 山羊流产嗜衣原体广东株的鉴定及分子特征分析[J]. 中国人兽共患病学报, 2016, 32(1): 17-20.
WANG Ling-ling, LIU Yong-jie, ZHENG Hai-xue, ZHANG Ke-shan, LIU Xiang-tao. Identification and molecular characteristic of Chlamydophila abortus Guangdong strain in goats. Chinese Journal of Zoonoses, 2016, 32(1): 17-20.
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