Establishment and evaluation of colloid gold labeled immuno chromatographic strip test for rapid diagnosis of human fascioliasis gigantica
ZHOU Yan1, XU Xue-nian1, CHENG Na1, LIU Yu-hua2, KONG Ling-ying3, YAO Kai-ling3, SHI Feng1, ZHANG Jian-guo4, Peter CHUN3, LUO Jia-jun2
1.National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention/Key Laboratory of Parasite and Vector Biology, MOH / WHO collaborating Centre for Tropical Diseases, Shanghai 200025, China; 2.Dali Prefecture Institute of Schistosomiasis Prevention and Control, Dali 671000, China; 3.EASE-Medtrend Biotech (Shanghai), LTD, Shanghai 201206, China; 4.Schistosomasis Control Station of Binchuan County, Binchuan 671600, China);
Abstract：To establish and evaluate a colloid gold-immunochromatographic assay (GICA) dynamic flow strip for the diagnosis of human fascioliasis, total RNA was prepared from Fascioliasis gigantica (F. gigantica) collected from Dali, Yunnan Province, China. FgCL1-YN gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR). The PCR product was sequenced and cloned into pET28a(+) vector. The recombinant plasmid was expressed and induced by isopropyl-β-D-thiogalactopyranoside (IPTG) to obtain recombinant protein, rFgCL1-YN. The mouse anti-human IgG4 monoclonal antibodies was conjugated with colloid gold as detecting reagent; the rFgCL1-YN and goat anti-mouse IgG antibody were immobilized on nitrocellulose in proper position as test line and control line, separately. The prepared immunochromatographic strip was evaluated using serum samples from fascioliasis gigantica patients (30 cases), schistosomiasis japonica patients (15 cases) and healthy donors (32 cases). Sensitivity detected by the GICA strip test was 100% (30/30). One serum sample from a healthy control was an invalid result with the GICA. Reference sera from schistosomiasis were all negatives for anti- F. gigantica IgG4 antibodies using the GICA. The sensitivity, specificity and total diagnostic coincidence rate of rFgCL1-YN-based the enzyme-linked immunosorbent assay (ELISA) was 100% (30/30), 100% (47/47) and 100% (77/77) respectively. Comparison between the GICA and the ELISA was made by exact probabilities in 2×2 table analysis. The P value of the total diagnostic coincidence rate, sensitivity and specificity was 1, 0.5 and 0.5, respectively (P>0.05). High degree of agreement was observed between the GICA and ELISA. The detection time of GICA was 10 min, and the serum concentration was less than 10 μL with highly sensitive. The developed immunochromatographic strip test using recombinant FgCL1-YN antigen as coated antigen is a sensitive, simple and rapid assay for diagnosing human fascioliasis.
周岩, 许学年, 程娜, 刘榆华, 孔令颖, 姚恺龄, 石峰, 张建国, 罗家军. 巨片形吸虫病快速诊断胶体金免疫层析试条的研制与评价[J]. 中国人兽共患病学报, 2016, 32(2): 114-118.
ZHOU Yan, XU Xue-nian, CHENG Na, LIU Yu-hua, KONG Ling-ying, YAO Kai-ling, SHI Feng, ZHANG Jian-guo, Peter CHUN, LUO Jia-jun. Establishment and evaluation of colloid gold labeled immuno chromatographic strip test for rapid diagnosis of human fascioliasis gigantica. Chinese Journal of Zoonoses, 2016, 32(2): 114-118.
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2016, Vol. 32 
