Abstract:We investigated the infection status of nanobacteria on patients of gallstone, and evaluated the clinical value of PCR by different primers. The nanobacteria from 30 cases of stone specimens and 43 cases of bile samples were detected by culture and subsequent indirect immunofluorescence staining(CIIFS), transmission electron microscopy(TEM) and polymerase chain reaction(PCR). Results showed that the positive rates of primers 1, 2 and culture and subsequent indirect immunofluorescence staining were 56.1%, 86.4% and 54.5% respectively. PCR analysis of primers 1 had a higher specificity for the detection of NB in patients with gallstone, and a high consistency with the results of CIIFS. There was no statistically significant difference (P>0.05) between primers 1 and CIIFS. The sensitivity of PCR for the detection of NB compared with the CIIFS method was 75.0%, with a specificity of 66.7%. The positive predictive value was 73.0%, and the negative predictive value was 69.0%. Primer 1 positive rate in the original group and the culture group were 29.6% and 50% respectively (P<0.05), after the culture can significantly improve the detection rate of nanobacteria. Primer 1's positive rate in the gallstone group and the bile group were 46.7% and 63.9% respectively (P>0.05). Detection of nanobacterial infection by primer 1 of PCR in gallstone has a high consistency with CIIFS and offers significant advantages for the rapid and reliable detection of nanobacterial infection in gallstone.
叶宁, 王晶晶, 朱明利, 刘寿荣. 不同PCR引物检测胆囊结石中纳米细菌效果的研究[J]. 中国人兽共患病学报, 2016, 32(2): 173-176.
YE Ning, WANG Jing-jing, ZHU Ming-li, LIU Shou-rong. Value of different PCR primers for detecting nanobacteria in gallstone. Chinese Journal of Zoonoses, 2016, 32(2): 173-176.
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