Genetic identification and sequene analysis for high-risk group and animals of Anaplasma phagocytophilum infection in some areas of Fujian Province,China
XIAO Fang-zhen1, LI Dan-ping2, XU Guo-ying1, CHEN-Yang1, DENG Yan-qin1
1.Fujian Center for Disease Control and Prevention /Fujian Priority Laboratory for Zoonoses, Fuzhou 350001, China; 2.Fujian Medical University, Fuzhou 350001, China
Abstract：To explore the nature infection and gene characteristics of Anaplasma phagocytophilum (A.phagocytophilum) in high-risk groups and animals of Fujian Province, China, blood samples from high-risk groups and animals were collected in Yongchun and Shanghang regions, for which nest polymerase chain reaction (PCR) was used to amplify the fragments of 16s rRNA gene of A. phagocytophilum. In addition, the positive PCR products were sequenced and compared with the corresponding sequences acquired by biological analysis software. The infection rate of ox, goats and dogs in Yongchun region were determined to be 53.85%, 24.71% and 12.50%, and the infection rates of goats and high-risk groups in Shanghang region were 53.06% and 8.33%, respectively. The nucleotide sequence homologies of partial 16s rRNA gene for both the objective and reference sequences concurrently reached up to 97%-100%. It was concluded that A. phagocytophilum was found in some regions of Fujian Province and it should be paid more attention in the following years.
肖方震, 李丹萍, 徐国英, 陈阳, 邓艳琴. 福建部分地区人和动物嗜吞噬细胞无形体基因检测和序列分析[J]. 中国人兽共患病学报, 2016, 32(3): 262-265.
XIAO Fang-zhen, LI Dan-ping, XU Guo-ying, CHEN-Yang, DENG Yan-qin. Genetic identification and sequene analysis for high-risk group and animals of Anaplasma phagocytophilum infection in some areas of Fujian Province,China. Chinese Journal of Zoonoses, 2016, 32(3): 262-265.
[1] Dumler JS. Anaplasma and Ehrlichia infection[J].Ann N Y Aead Sci, 2005, 1063(12): 361-373. [2] Chen SM, Dumler JS, Bakken JS, et al. Identification of agranulocytotropic Ehrlichia species as the etiologic agent of human disease[J]. J Clin Microbiol, 1994, 32(3): 589-595. [3] Jiang LP, Zheng SG, Wang ZG, et al. Detection and sequence analysis of Ehrlichia and Anplasma DNA from rats in Jindong District[J]. Zhejiang Prev Med, 2008, 20(1): 1-3,6. (in Chinese) 姜理平,郑寿贵,王志刚,等.金东区鼠类检出埃立克体及无形体DNA 片断[J].浙江预防医学,2008,20(1):1-3,6. [4] Bian CL, Gong ZD, Zhang LY, et al. Identification of Anaplasma phagocytophilum in small mammals from Hengduan Mountains of Southwest China[J]. Chin J Epidemiol, 2009, 30(12): 1277-1280. (in Chinese) 边长玲,龚正达,张丽云,等.中国西南横断山区小型兽类嗜吞噬细胞无形体基因的检测及序列测定[J].中华流行病学杂志,2009,30(12):1277-1280. [5] Zhan L, Cao WC, Jiang JF, et al. Anaplasma phagocytophilumin livestock and small rodents[J]. Vet Microbiol, 2010, 144(3-4): 405-408. [6] Shi YL, Wang SW, Hu WF, et al. Genetic identification and sequence analysis of the 16S rRNA Genes of Anaplasma phagocytophilum in Anhui Province,China[J]. Anhui J Prev Med, 2013, 19(5): 329-338. (in Chinese) 史永林,王誓闻,胡万富,等.安徽地区家畜嗜吞噬细胞无形体16S rRNA 基因检测和序列分析[J].安徽预防医学杂志,2013,19(5):329-338. [7] Zhao QL, Huang L, Yang X, et al. 16srRNA sequence analysis of Anaplasma and Ehrlichia in rodents from parts area of Xinjiang[J]. Chin J Zoonoses, 2013, 29(8): 743-747. (in Chinese) 赵庆亮,黄林,杨霞,等. 新疆部分地区鼠类无形体和埃立克体的调查及16srRNA序列分析[J]. 中国人兽共患病学报,2013,29(8):743-747. [8] Zheng BF, Ying KM, Zhang MT, et al. Investigation for Anaplasma phagocytophilum infection in rodents in Pan'an region, Zhejiang Province[J]. Chin J Zoonoses, 2013, 29(3): 274-277. (in Chinese) 郑柏福,应凯满,张孟田,等. 浙江磐安地区啮齿动物嗜吞噬细胞无形体感染研究[J]. 中国人兽共患病学报,2013,29(3):274-277.
