Sequence analysis for E genes of autochthonous dengue virus isolates from Nanping, Fujian Province, 2014
WANG Jin-zhang1, CHEN Hong-bin1, ZHANG Yong-jun1, WENG Yu-wei1, WU Chun-min2, ZHENG Kui-cheng1, YAN Yan-sheng1
1.Fujian Provincial Center for Disease Control and Prevention,Fujian Priority Laboratory for Zoonoses, Fuzhou 350001, China; 2.Nanping Center for Disease Control and Prevention, Nanping 353000, China
Abstract：In September 2014, an autochthonous outbreak of dengue fever emerged in Nanping, Fujian Province. In order to identify serotypes of the outbreak-related dengue viruses and track probable transmission sources, serum samples of patients at acute phases were inoculated on C6/36 cell lines. Serotypes were initially tested by reverse transcriptase polymerase chain reaction (RT-PCR), full-length fragments of E gene were amplified by RT-PCR, and were sequenced. A phylogenetic tree was drawn by neighbor-joining (NJ) method. Among the four DENV-1 strains and two DENV-2 strains isolated during the outbreak, it was indicated from BLAST analyses of sequence data that DENV-1 strains showed 99.7% sequence homology with strain D13459 (Donguang) from Guangdong in 2013, and that DENV-2 strains were identical to strain DENV2/CN/GZ05/2014 from Guangdong in 2014. Phylogenetic analysis also indicated that outbreak-associated DENV-1 strains were classified as genotype Ⅴ and DENV-2 strains were assigned to genotype Ⅳ. Therefore, it was suggested from epidemiologic and molecular evidence that DENV-1 viruses might originate from Guangdong or India, and that DENV-2 viruses might be introduced from Guangdong or Singapore.
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2016, Vol. 32 
