Identification and antimicrobial resistance of pathogenic Aeromonas hydrophila
GAO Zhi-ling1, 2, JI Xue2, 3, CHEN Ping1, GUO Xue-jun2, 3, LIU Jun2, 3, ZHU Ling-wei2, 3, ZHOU Wei2, 3, FENG Shu-zhang2, 3, SUN Yang2, 3
1. College of Food Science and Technology, Jilin Agricultural University, Changchun 130118, China; 2. Institute of Military Veterinary, AMMS, Changchun 130122, China; 3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, China
Abstract：The occurrence of antimicrobial resistance and prevalent distribution of pathogenic Aeromonas hydrophila from aquatic products was investigated in this study. A total of 279 strains were isolated from 319 samples from commercially available freshwater fish and aquaculture farms in Changchun area, in which virulence genes were detected by PCR assays. Among them, 24 strains were positive (8.06%). Most of them carry both genes of hemolysin (hly) and genes of aerolysin (aerA). Biological characteristics and drug resistance spectrum of the pathogenic Aeromonas hydrophila isolates were characterized and identified. The 24 strains could cause β-hemolytic with positive protease test. To evaluate cytotoxicity by vero cell lines, the culture supernatant of the 24 strains resulted in severe cytopathogenic effect. All of pathogenic Aeromonas hydrophila isolates were resistant to at least 4 kinds of antibiotics. The results showed that all of the pathogenic strains had multiple resistance patterns. The investigation provided basic data for preventing and controlling the transmission of Aeromonas hydrophila with the multi-drug resistant.
[1] Shen JY. Research progress on Aeromonas hydrophila [J]. J Zhejiang Ocean Univ, 2008, 27(1): 78-86. doi:10.3969/j.issn.1008-830X.2008.01.017 (in Chinese) 沈锦玉. 嗜水气单胞菌的研究进展[J]. 浙江海洋学院学报, 2008, 27(1): 78-86. [2] Coelwll RR, Macdonell MT, Deley J. Proposal to recognize the family Aeromonadaceae fam. Nov[J]. Intl J Systemic Evolutionary Microbiol, 1986, 36: 473-477. doi:10.1099/00207713-36-3-473 [3] GB/T 18652-2002,State Standard of the People's Republic of China[S].Beijing,Standards Press of China,2002. GB/T 18652-2002, 中华人民共和国国家标准[S].北京:中国标准出版社,2002. [4] Janda JM, Duffey PS. Mesophilic aeronaonadsin human disease: current taxonomy laboratory identification and infectious disease spectrum[J]. Rev Infect Dis, 1998 (100): 980-997. doi:10.1093/clinids/10.5.980 [5] Chen MJ, Wang H. Continuous surveillance of antimicrobial resistance among nosocomial gram-negative bacilli from intensive care units in China[J]. Natl Med J China, 2003, 83(5): 375-381. doi:10.3760/j:issn:0376-2491.2003.05.007 (in Chinese) 陈民钧, 王辉. 中国重症监护病房革兰阴性菌耐药性连续7年监测研究[J]. 中华医学杂志, 2003, 83(5): 375-381. [6] Cai LJ, Xu BQ, Lin QC. Comparison and analysis of drug resistance to morbific bacterium aeromonas hydrophila isolated from aquatic animals[J]. Fisheriers Sci, 2011, 30(1): 42-45. doi:10.3969/j.issn.1003-1111.2011.01.009 (in Chinese) 蔡丽娟, 许宝青, 林启存. 水产致病性嗜水气单胞菌耐药性比较与分析[J]. 水产科学, 2011, 30(1): 42-45. [7] Vivekanandhan G, Savithamani K, Mohamed HA. et a1. Antibiotic resistance of Aeromonas hydrophila isolated form marketed fish and prawn of South India[J]. Intl J Food Mierobiol, 2002, 76(1/2): 165-168. doi:10.1016/S0168-1605(02)00009-0 [8] Wang GH, Clark CG, Liu CY, et al. Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR[J]. J Clin Microbiol, 2003, 41(3): 1048-1054. doi:10.1128/JCM.41.3.1048-1054.2003 [9] Dorsch M, Ashbolt NJ, Cox PT, et al. Rapid identification of Aeromonas species using 16S rDNA targeted oligonucleotide primers: a molecular approach based screening of environmental isolates[J]. Appl Bacteriol, 1994, 7: 722-726. doi:10.1111/j.1365-2672.1994.tb02825.x [10] Alperi A, Figueras MJ, Inza I, et al. Analysis of 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation[J]. Intl Microbiol, 2008, 11(3): 185-194. doi:10.2436/20.1501.01.59 [11] Clinical and Laboratory Standard Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria: M45A-A2[M]. Wayne: Clinical and Laboratory Standards Institute, 2010: 12-13. [12] Wu LZ, Zhou HL, Pan MY, et al. Analysis of diarrhea caused by Aeromonas hydrophila [J]. Chin J Nosocomiol, 2000, 10(4): 256-258. doi:10.3321/j.issn:1005-4529.2000.04.039 (in Chinese) 吴龙章, 周辉林, 潘美玉, 等. 嗜水气单胞菌致腹泻分析[J].中华医院感染学杂志. 2000, 10(4): 256-258. [13] Meng S, Ye CY. Novel triplex real-time TaqMan PCR assay for the detection of Aeromonas hydrophila [J]. Chin J Zoonoses, 2012, 28(3): 217-222. doi:10.3969/j.issn.1002-2694.2012.03.006 (in Chinese) 孟双, 叶长芸. 嗜水气单胞菌实时荧光三重TaqMan PCR快速检测体系的建立[J]. 中国人兽共患病学报, 2012, 28(3): 217-222. [14] Ko WC, Yu KW, Liu CY, et al. Increasing antibiotic resistance in clinical isolates of Aeromonas strains in Taiwan[J]. Antimicrob Agents Chemother, 1996, 40(5): 1260-1262. [15] Qu F, Bao CM, Cui EB, et al. Prevalence and antimicrobial resistance of different Aeromonas species[J]. Chin J Infect Chemother, 2004, 4(5); 302-305. doi:10.3321/j.issn:1009-7708.2004.05.014 (in Chinese) 曲芬, 鲍春梅, 催恩博, 等. 气单胞菌不同种的流行及耐药性[J]. 中国抗感染化疗杂志, 2004, 4(5); 302-305. [16] Zhu XZ, He HJ, Deng SZ, et al. An analysis of the antibiotic resistance and plasmid profile Aeromonas hydrophila isolated from Jiangxi Province[J]. Acta Agriculturae Universitatis Jiangxiensis, 2012, 34(6): 1262-1268. doi:10.3969/j.issn.1000-2286.2012.06.031 (in Chinese) 朱秀芝, 何后军, 邓舜洲, 等. 嗜水气单胞菌江西地区分离株耐药性及耐药质粒分析[J]. 江西农业大学学报, 2012, 34(6): 1262-1268. [17] Fang YF, Pan XY, Lin LY, et al. Molecular mechanisms of quinolone resistance in Aeromonas hydrophilia [J]. Acta Microbiologica Sinica, 2014, 54(2): 174-182. doi:10.13343/j.cnki.wsxb.2014.02.006 (in Chinese) 方一风, 潘晓艺, 蔺凌云, 等. 嗜水气单胞菌对喹诺酮类药物耐药的分子机制[J]. 微生物学报, 2014, 54(2): 174-182. [18] Hong J, Pan LD. Analysing plasmid fingerprinting and relationship between plasmids and resistance in Aeromonas hydrophila from fish[J]. J Fisheries China, 2010, 34(12): 1908-1916. doi:10.3724/SP.J.1231.2010.06908 (in Chinese) 洪经, 潘连德. 鱼源嗜水气单胞菌质粒的指纹图谱及其与耐药性的关系[J]. 水产学报, 2010, 34(12): 1908-1916. [19] Saavedra MJ, Guedes NS, Alves A, et al. Resistance to β-lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss)[J]. Intl Microbiol, 2004, (7): 207-211. [20] Si LN, Li SW, Wang D, et al. Drug sensitivity test on fifteen strains of pathogenic Aeromonas hydrophila from Northeast China[J]. Acta Agriculturae Universitatis Jiangxiensis, 2011, 33(4): 786-790. doi:10.3969/j.issn.1000-2286.2011.04.029 (in Chinese) 司力娜, 李邵戊, 王荻, 等. 东北三省15株致病性嗜水气单胞菌分离株的药敏实验分析[J]. 江西农业大学学报, 2011, 33(4): 786-790. [21] Li AH, Cai TZ, Wu YS, et al. Inverstigation on drugresistance of fish bacterial pathogen- Aeromonas hydrophila in China[J]. Microbiol, ogy2001, 28(1): 58-63. doi:10.3969/j.issn.1000-2286.2011.04.029 (in Chinese) 李爱华, 蔡桃珍, 吴玉深, 等. 我国鱼类病原-嗜水气单胞菌的耐药性研究[J]. 微生物学通报, 2001, 28(1): 58-63. [22] Wang JB, Xu LP, Wang XL, et al. Study on the antibiotic resistance in Aeromonas hydrophila isolated from diseased fish in Beijing[J]. Beijing Agr, 2012(30): 77-80. (in Chinese) 王静波, 徐立浦, 王小亮,等. 北京地区养殖鱼类来源嗜水气单胞菌耐药性研究[J]. 北京农业, 2012 (30): 77-80. [23] Wang YJ, Li RF, Li H, et al. Molecular epidemiology studies on Aeromonas hydrophila strains isolated from fresh water fish in Liaoning Province[J]. J Agr Sci Technol, 2012, 14(4): 128-134. doi:10.3969/j.issn.1008-0864.2012.04.19 (in Chinese) 王友娟, 李荣峰, 李华, 等. 辽宁地区养殖淡水鱼感染嗜水气单胞菌的流行病学调查[J]. 中国农业科技导报, 2012, 14(4): 128-134.
2016, Vol. 32 
