Protective immunity and mechanism of dendritic cell multivalent nucleic acid vaccine against infection of Schistosoma japonicum
SHEN Ding-wen1, LUO Jin-ping1, WANG Ruo-yu1, XU Pei-pei1, DAI Bo1, YU Jun-lin1, LI Yong-long2
1.Basic Medical College, Hubei University of Science and Technology, Xianning 437100, China; 2. Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract：To study the effect of gene transfer on the functions of dendritic cell (DC) and protective immunity and mechanism of DC multivalent nucleic acid vaccine against infection of Schistosoma japonicum, DCs were transfected with recombinant eukaryotic expression vector pcDNA3-Sj26, pcDNA3-Sj23 and pcDNA3-Sj14. The ability of DC to absorb antigen was determined by flow cytometry (FCM). The mixed lymphocyte reaction (MLR) was used to detect the effect of gene transferred DC on stimulating allogenetic T lymphocyte. BALB/c mice were immunized three times with Sj26, Sj23 and Sj14 gene transferred DC, alone or combination, and infected with 40 cercariae of S. japonicum per mouse 2 weeks after the last immunization. The number of adult worm and the egg number in liver were calculated six weeks after infecting. ELISA was used to detect the levels of specific IgG, IFN-γ and IL-4 in sera from each mice group. The level of IFN-γ and IL-4 in the culture supernatant of spleen lymphocytes stimulated with soluble egg antigen (SEA) and ConA were quantified by ELISA. The proliferation of spleen lymphocytes were measured by the method of MTT. Compared with pcDNA3 transferred DC and untreated DC, the fluorescence intensity of antigen absorbing decreased significantly in S. japonicum encoding gene-transferred DC (P<0.01), and the stimulating index (SI) increased significantly (P<0.01). The rate of worm reduction and egg reduction in each group of immunization were higher than that of control group (P<0.01), while protective immunity induced by gene transfer DC were significantly higher than that of gene transfer DC alone (P<0.001).Compared with previous immunization, the levels of specific IgG increased significantly in sera from group of S. japonicum encoding gene-transferred DC 2 weeks after the last immunization (P<0.05), the levels of IFN-γ increased significantly (P<0.01), while the levels of IL-4 were not significantly different. In response to ConA and SEA, the level of IFN-γ in the culture supernatant of spleen lymphocytes from group of S. japonicum encoding gene-transferred DC increased significantly, the level of IL-4 decreased significantly, while SI increased significantly (compared with control group, P<0.001). Results indicated that S. japonicum encoding gene transfer can promote DC maturation and enhance the biologic activity of DC. DC multivalent nucleic acid vaccine could induce and enhance protective immunity against infection of S. japonicum. Predominant Th1 type immune response might play an important role in the protective immunity induced by gene-transferred DC against infection of S. japonicum.
沈定文, 罗金萍, 王若愚, 许培培, 戴波, 余军林, 李雍龙. 树突状细胞多价核酸疫苗抗血吸虫感染作用及机制研究[J]. 中国人兽共患病学报, 2016, 32(4): 406-411.
SHEN Ding-wen, LUO Jin-ping, WANG Ruo-yu, XU Pei-pei, DAI Bo, YU Jun-lin, LI Yong-long. Protective immunity and mechanism of dendritic cell multivalent nucleic acid vaccine against infection of Schistosoma japonicum. Chinese Journal of Zoonoses, 2016, 32(4): 406-411.
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