四川动物源大肠杆菌耐药性、产β-内酰胺酶及血清型的检测

doi:10.3969/j.issn.1002-2694.2016.04.018

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摘要

目的为了解四川地区动物源大肠杆菌临床分离菌株耐药性,产β-内酰胺酶以及产酶菌株血清型流行规律。方法采用微量肉汤稀释法对467株临床菌株进行了27种抗菌药物的耐药性检测;采用CLSI推荐产ESBLs、AmpC和碳青霉烯酶检测方法检测了所有菌株产β-内酰胺酶情况;采用O抗原鉴定法对产酶菌株血清型进行了鉴定。结果 467株菌对27种抗生素产生了不同程度耐药性;菌株产ESBLs、AmpC和碳青霉烯酶检出率分别为36.83%、13.70%和0.00%,产酶菌比非产酶菌具有更严重的耐药性;176株产酶菌共检测出31种血清型,以O₁₃₁、O₁₀₇、O₇₈、O₉、O₁₂₇、O₂₀和O₁₅₇为优势血清型。结论本次检测大肠杆菌ESBLs和AmpC检出率较高,耐药形势严重,血清型复杂,应加强动物源大肠杆菌产β-内酰胺酶及血清型的监测。

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	代鹏飞
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	邓林

关键词 ：大肠杆菌, 耐药性, β-内酰胺酶, 血清型

Abstract：

We investigated epidemiological characteristics of resistance, β-lactamase-producing and serotype of E. coli isolates in animals. Broth microdilution method was used to detect antimicrobial resistance; ESBLs, AmpC and carbapenemase production tests were performed on all isolates; O-antigen identification method was used to detect the serotypes of β-lactamase positive strains. The 467 isolates indicated different resistance to both β-lactam and non β-lactam; ESBLs, AmpC and carbapenemase were detected with the rates of 36.83%, 13.70% and 0.00%, respectively. β-lactamase positive isolates showed more serious resistance than negative isolates; 176 β-lactamase positive isolates belonged to 31 serotype and the major epidemic serotype were O₁₃₁, O₁₀₇, O₇₈, O₉, O₁₂₇, O₂₀ and O₁₅₇. The detection rate of ESBLs and AmpC was high in the isolates tested, the antibiotic resistance situation was serious, and the serotype of β-lactamase positive isolates was diversified, the surveillance of β-lactamase-producing and serotype of E. coli must be reinforced.

Key words： E. coli resistance β-lactamase serotype

收稿日期: 2015-07-20

PACS:

R378.2

通讯作者: 林居纯,Email: juchunlin@126.com

引用本文:

代鹏飞, 舒刚, 林居纯, 钟钦卿, 邓林. 四川动物源大肠杆菌耐药性、产β-内酰胺酶及血清型的检测[J]. 中国人兽共患病学报, 2016, 32(4): 412-416. DAI Peng-fei, SHU Gang, LIN Ju-chun, ZHONG Qin-qing, DENG Lin. Surveillance of resistance, β-lactamase-producing and serotypes of E. coli isolates from animals in Sichuan Province, China. Chinese Journal of Zoonoses, 2016, 32(4): 412-416.

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http://www.rsghb.cn/CN/10.3969/j.issn.1002-2694.2016.04.018 或 http://www.rsghb.cn/CN/Y2016/V32/I4/412

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