Comparative proteomic research between the Streptococcus suis serotype 2 Chinese highly virulent strain and the covR isogenic mutant
NI Hua1,2, ZHANG Jian1,2, ZHENG Feng2, HU Dan2, LI Xian-fu2, WANG Chang-jun2, PAN Xiu-zhen1,2
1. College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; 2.Institute of Military Medical Sciences, Nanjing Command, Nanjing 210002, China
Abstract：In order to search for the virulence factor regulated by CovR, the proteomics of the whole-cell protein were compared between Streptococcus suis serotype 2 wild strain 05ZYH33 and an isogenic mutant strain △covR by two-dimensional gel electrophoresis. The 05ZYH33 and △covR were cultured in Todd-Hewitt Broth medium then the whole-cell proteins sample were extracted. The 2-DE gel was conducted using the pH3-10 IPG strip for the first dimension IEF and followed by SDS-PAGE. After electrophoresis, the gels were stained and analyzed. Results showed that the 05ZYH33 and △covR had 559 and 491 protein spots respectively. Compared with the 05ZYH33, the mutant strain had 40 proteins more than 3 folds changed which identified 15 proteins by mass spectrum. Those proteins major involved in cell metabolic enzymes, such as adenylate kinase, glutamate dehydrogenase and PTS system components, etc., as well as molecular chaperone proteins GroEL and Dnak. The △covR had about 124 specific protein spots in which 15 proteins were identified by mass spectrum. Those proteins were participate in the cell process of sugar metabolism, such as phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase, etc. Beside of those 5 specific proteins of 05ZYH33 was identified by mass spectrum. Appraisement of 35 differentially expressed proteins involved in bacterial virulence, host cell adhesion and cell division, etc., and the molecular chaperone up-regulated expression showed that the regulation may be occurred in the profile of protein modification. These results provided better understanding on pathogenic mechanisms of Streptococcus suis type 2 at the level of protein expression.
倪华, 张剑, 郑峰, 胡丹, 李先富, 王长军, 潘秀珍. 2型猪链球菌中国强毒株及其covR基因突变株的蛋白质组学研究[J]. 中国人兽共患病学报, 2016, 32(5): 417-423.
NI Hua, ZHANG Jian, ZHENG Feng, HU Dan, LI Xian-fu, WANG Chang-jun, PAN Xiu-zhen. Comparative proteomic research between the Streptococcus suis serotype 2 Chinese highly virulent strain and the covR isogenic mutant. Chinese Journal of Zoonoses, 2016, 32(5): 417-423.
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2016, Vol. 32 
