Isolation and identification of HEV71 strains from children with hand-foot-and-mouth disease in different conditions and analysis of their VP1 genotypes
JIANG Zhuo-jing1,2, HE Ting-ting2, CHEN Jin-kun2, YAN Jie1
1. Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine,Hangzhou 310058, China; 2. Shaoxing Center for Disease Control and Prevention, Shaoxing 312071, China
Abstract：The aim of this study was to isolate the human enterovirus 71 (HEV71) strains from the hand-foot-and-mouth disease (HFMD)-suffering children with different pathogenetic conditions, and the VP1 genotypes of the viral isolates and the mutation of drug-binding sites in the VP1 genes were amalyzed. Firstly, HEV71 strains were isolated from the samples of four mild and four severe HFMD children, and then the viral isolates were identified by RT-PCR. The entire VP1 genes in the isolated HEV71 strains were amplified by RT-PCR for sequencing. Using professional bioinformatic softwares, the VP1 genotypes of the HEV71 isolates and the mutation of drug-binding sites in the VP1 genes were determined, and then the VP1 gene-based phylogenetic evolution tree of these viral isolates was generated. The results showed that HEV71 strains could be isolated from all the samples of the eight cases. Compared with the reported VP1-C4a genotype of HEV71, the identities of nucleotide and amino acid sequences of the viral isolates were 97.2%-98.8% and 99.3%-99.7%, respectively. The mutation at 179th drug-binding site in the VP1 genes (V179L) from two of the four severe cases was found. Five or three of the eight isolates presented the higher genetic relationship with those from Anhui and Hunan, or Shanghai and Shandong areas. All the results led to the conclusions that all the HEV71 strains isolated from the HFMD children in the local area with different pathogenetic conditions belong to VP1-C4a genotype, and the mutation at 179th drug-binding site may be associated with severity of the disease.
蒋卓婧, 何婷婷, 陈金堃, 严杰. 轻或重症手足口病患儿HEV71分离鉴定及其VP1基因型分析[J]. 中国人兽共患病学报, 2016, 32(5): 429-434.
JIANG Zhuo-jing, HE Ting-ting, CHEN Jin-kun, YAN Jie. Isolation and identification of HEV71 strains from children with hand-foot-and-mouth disease in different conditions and analysis of their VP1 genotypes. Chinese Journal of Zoonoses, 2016, 32(5): 429-434.
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2016, Vol. 32 
