Surveillance and analysis of the pathogens of viral gastroenteritis in 2011 and 2013,Wuhan,China
PANG Bei-bei1, ZHOU Xuan1, Nobumichi Kobayashi2, PENG Jin-song1, WANG Yuan-hong*
1 Wuhan Centers for Disease Prevention and Control, Hubei 430015, China; 2 Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan
Abstract：To survey and analyze the pathogens of viral gastroenteritis among infants and adults in 2011 and 2013 in Wuhan, the stool samples were detected for the rotavirus, norovirus, astrovirus and sapovirus by polyacrylamide gel electrophoresis (PAGE), RT-PCR and PCR. The nucleotide sequences of partial positive samples were determined and their identities were analyzed by BLAST and/or genotyping tool online. The detection rates of rotavirus, norovirus, astrovirus, sapovirus were 21.6%(296/1 368), 35.7%(488/1 368),10.2%(140/1 368) and 7.7% (105/1 368),respectively. The detection rate of adenovirus in children was 8.2% (70/853).The detection rates of norovirus and rotavirus were higher in 13-24 months and 7-24 months age groups, respectively, and 40-59 years old age group. The detection rate of rotavirus was higher in autumn and winter. Astrovirus, sapovirus and adenovirus were more frequently detected in children under 2 years old. Seasonality was not observed in astrovirus, sapovirus or adenovirus. The genotypes of 196 group A rotavirus were determined. Of these, the most frequent were G9P[8](40.82%), followeing by G1P[8](26.53%), G3P[8](16.33%)and G2P[4](11.73%). The genotypes of norovirus were GII 4(7/12) and GII 3(5/12), and that of astrovirus were type 1(8/13),type 8(4/13) and type 5(1/13). The genotypes of sapovirus were GI.1(2/6), GI.2 (2/6) and GII.1 (2/6). The main pathogens of viral gastroenteritis were norovirus and rotavirus in 2011 and 2013, in Wuhan.
庞蓓蓓, 周璇, 彭劲松, 王远虹. 2011年和2013年武汉市病毒性胃肠炎病原监测与分析[J]. 中国人兽共患病学报, 2016, 32(5): 457-461.
PANG Bei-bei, ZHOU Xuan, Nobumichi Kobayashi, PENG Jin-song, WANG Yuan-hong. Surveillance and analysis of the pathogens of viral gastroenteritis in 2011 and 2013,Wuhan,China. Chinese Journal of Zoonoses, 2016, 32(5): 457-461.
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