Survey on rabies viruses in rabies patients and dog population from endemic sites of Yunnan Province, China and the viral M gene sequence analysis
DING Ji-chao1, 2, ZHANG Yu-zhen2, LI Hao3, YANG Wei-hong2, FENG Yun2, TAO Xiao-Yan3, SHEN Xin-xin3, TANG Qing3, ZHANG Hai-lin2
1. School of Public Health, Dali University, Dali 671000, China; 2. Yunnan Institute of Endemic Diseases Control and Prevention / Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali 671000, China; 3. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract：We conducted investigation on the rabies virus (RABV) infected from rabies patients and dog population in endemic sites, Yunnan Province, China and the viral matrixprotein (M) gene sequence analysis, providing references for control and prevention of rabies. A total of 606 brain tissue samples of dogs collected from endemic sites, as well as 8 saliva and 1 cerebrospinal fluid samples of rabies patients collected from Yunnan Province in 2008-2009. RABV antigen and nucleic acid were detected by direct immunofluorescence assay and RT-PCR respectively, and positive samples were sequenced for the M gene.According to the nucleotide and amino acid of target gene, the genetic homology and the phylogeny were analyzed. Above specimens tested, 16 specimens were RABV antigen and/or nucleic acid positive. Among them, 3.10% (10/323) were from apparently healthy dog brain specimens of endemic sites, 5 saliva and 1 cerebrospinal fluid were from human rabies cases. In addition, 283 dog brain specimens collected from dog meat restaurant were negative. The M gene sequence of the 16 strains have been acquired, the rate of their nucleotide homology and deduced amino acid homology were 88.5%-100% and 85.2%-99.5% respectively. Compared with aG and CTN181 strains (vaccine strains for humans in China) nucleotide (deduced amino acid) homology were 83.9%-85.7% (82.3%-93.6%) and 99%-99.7% (98.5%-99%) respectively. The phylogenic analysis indicated that the 16 isolates from Yunnan Province divided into I and II phylogenic subgroups. These 2 phylogenic subgroups of RABV from Yunnan Province have a closer genetic relationship with the street strains isolated from Southeast Asia and neighboring provinces of China. Rabies epidemic in Yunnan Province might be attributed to rabies spread from Southeast Asia and neighboring provinces. Some apparently healthy dogs can infected RABV. The M gene nucleotide and amino acid of Yunnan strains of rabies virus have a higher homology and a closer genetic relationship with CTN strain of human rabies vaccine used in China, but they with aG strains of human rabies vaccine used in China homologous exist obvious difference.
丁继超, 章域震, 李浩, 杨卫红, 冯云, 陶晓燕, 申辛欣, 唐青, 张海林. 云南省狂犬病病人和犬类感染狂犬病病毒及M基因序列分析[J]. 中国人兽共患病学报, 2016, 32(6): 518-524.
DING Ji-chao, ZHANG Yu-zhen, LI Hao, YANG Wei-hong, FENG Yun, TAO Xiao-Yan, SHEN Xin-xin, TANG Qing, ZHANG Hai-lin. Survey on rabies viruses in rabies patients and dog population from endemic sites of Yunnan Province, China and the viral M gene sequence analysis. Chinese Journal of Zoonoses, 2016, 32(6): 518-524.
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2016, Vol. 32 
