Host cell injuries caused by 2Apro of EV71 detected by MTT assay
QIAO Qiao1, LI Jing1, ZHANG Wen-qiang2, MA Ying-wei1, ZHUANG Zhi-chao1, BAI Yong-juan1, ZHAO Li1, WANG Zhi-yu1, WEN Hong-ling1
1.Department of Virology, School of Public Health, Shandong University, Jinan 250012, China ; 2.Shandong Center for Disease Control and Prevention,Jinan 250014,China
Abstract:We studied host cell proliferations and injuries caused by 2Apro of EV71 in Vero cells, RD cells and U87 cells and to reveal the role of 2Apro in EV71 pathogenic mechanism. Vero cells, RD cells and U87 cells were infected by wild strains EV71 SDLY 107 (EV71 fatal strain), EV71 SDLY 1 (EV71 mild strain) and chimeric strain EV71 SDLY 107-2A-1, the survival of three cell lines at different time post infected by viruses were detected used by MTT assay, the cell growth curves were drew to compare cell proliferation and the survival rates of three cell lines at 48 hours post infected by different viruses were analyzed by ANOVA. Results showed that cell proliferations and survival rates of Vero cells and RD cells infected by EV71 SDLY 107 were lower than EV71 SDLY 1 (t=41.64,P<0.001;t=33.28,P<0.001). The proliferations and survival rates of Vero cells and RD cells post-infected by EV71 SDLY 107-2A-1 were higher than that in EV71 SDLY 107 and lower than that in EV71 SDLY 1 (t=17.97,P<0.001;t=42.09,P<0.001). The survival rate of U87 cells infected by EV71 SDLY 107-2A-1 was lower than that in EV71 SDLY 1 (t=8.85,P<0.001) and no different than that in EV71 SDLY 107 (t=0.74,P=0.603). U87 cells had the lowest survival rate and Vero cells had the highest survival rate at 48 h post infected by the same virus strain. The 2Apro of virulent strain caused more severe cell injury than 2Apro of attenuated virus, which reveals 2Apro plays a role in the effect of EV71 on host cells.
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