Abstract:The aim of this study was to establish mice model with Helicobacter suis (H. suis) infection and explore its significance. A total of 40 female C57BL/6 mice were randomly divided into model group and control group. The mice in model group were given 0.5 mL pig gastric mucosal homogenates containing H. suis by gavage, while the mice in control group were given the same amount of PBS. After 1 month and 3 months, half of each group were randomly sacrificed and their gastric mucosa were taken out for PCR and HE staining respectively to detect the H. suis colonization and the formation of lymphoid follicles in the stomach. Results showed that H. suis existed in pig gastric mucosal homogenates used for gavage and could colonize in the stomach of mice, which also existed at 3 months postinfection. The formation of gastric lymphoid follicles was detected in the stomachs of mice at 1 month and 3 months after H. suis formation, and the size of follicles at 3 months postinfection increased significantly compared with that at 1 month postinfection. In conclusion, a mouse model of H. suis infection was established successfully. H. suis infection can induce the formation of lymphatic follicles in C57BL/6 mice. Therefore it may play an important role in the development of gastric mucosa-associated lymphoid tissue (MALT) Lymphoma.
杨若明, 杨林, 田字彬, 于亚男, 毛涛, 李晓宇, 张帅庆. Helicobacter suis感染小鼠模型的建立及其意义[J]. 中国人兽共患病学报, 2016, 32(7): 604-607.
YANG Ruo-ming, YANG Lin, TIAN Zi-bin, YU Ya-nan, MAO Tao, LI Xiao-yu, ZHANG Shuai-qing. Establishment of mice model with Helicobacter suis infection and its significance. Chinese Journal of Zoonoses, 2016, 32(7): 604-607.
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