Molecular identification of tick species and sequence analysis of Ehrlichia in Lincang area of Yunnan Province,China
YA Hong-xiang, ZHANG Yun-zhi, WANG Jing-lin
Yunnan Institute of Endemic Diseases Control and Prevention/Yunnan Provincial Key Laboratory of Natural Focal Disease Control and Prevention,Dali 671000,China
Abstract:To investigate tick species and Ehrlichia infection among ticks in Lincang area of Yunnan province,42 ticks were captured from body surface of farm cattle and divided into 21 groups by morphological identification. Partial COI segments of ticks and 16S rRNA and groEL segments of Ehrlichia were amplified separately by polymerase chain reaction (PCR),and the homology were sequenced and analyzed with other known sequences. Among them,90.48% (38/42) ticks were identified as Boophilus microplus and 9.52% (4/42) ticks as Haemaphysalis by morphological identification. The 21 out of 21 groups samples were positive for ticks COI fragments (detection rate,100%),4 for Ehrlichia 16S rRNA fragments (detection rate,19.05%) and 4 for Ehrlichia groEL segments (detection rate,19.05%). Sequence analysis showed that 2 fragments of 21COI sequences and Haemaphysalis pava (JX573136) from Australia showed higher nucleotide homology,all reaching up to 89.3%. The 19 fragments of 21COI sequences and Boophilus microplus B3 from Malaysia showed higher nucleotide homology,all reaching up to 99.5%. Four Ehrlichia 16S rRNA fragments and E. chaffeensis Arkansas strains from USA,and E. chaffeensis BY-YQ-HME-O18 stains from Beijing of China,and E. ewingii Aa2FT349 strains from USA showed higher nucleotide homology,all reaching up to 100%. Four Ehrlichia groEL fragments and E. ssp. Yonaguni206 stains from Japan showed higher nucleotide homology,all reaching up to 93.9%. It’s confirmed by molecular identification that the epidemic Ehrlichia infected Boophilus microplus from body surface of farm cattle in Lincang area of Yunnan Province were similar to Japan Yonaguni 206 strains,in addition,a new local Haemaphysalis may exist in this region.
亚红祥, 张云智, 王静林. 云南临沧地区蜱种分子生物学鉴定及埃立克体基因序列分析[J]. 中国人兽共患病学报, 2016, 32(9): 793-797.
YA Hong-xiang, ZHANG Yun-zhi, WANG Jing-lin. Molecular identification of tick species and sequence analysis of Ehrlichia in Lincang area of Yunnan Province,China. Chinese Journal of Zoonoses, 2016, 32(9): 793-797.
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