Tick species and genetic variants analysis of tick gene in Hengduan Mountains, west Yunnan Province, China
YA Hong-xiang1, SHEN Shu2, SU Zheng-yuan2, DENG Fei2, ZHANG Yun-zhi1
1. Yunnan Institute of Endemic Diseases Control and Prevention /Yunnan Provincial Key Laboratory of Natural Focal Disease Control and Prevention, Dali 671000, China; 2. Wuhan Institute of Virology, China Academy of Science, Wuhan 430071, China
Abstract：To investigate the species composition and genetic variants of ticks in Hengduan Mountains of the western Yunnan Province, 1 874 individual ticks were captured from the body surface of domestic animals and were identified as four species of one genus of one family by species morpholog. Of them, 97.35% (1 637/1 874) of ticks were Rhipicephalus microplus, 11.63% (218/1 874) Rhipicephalus haemaphysaloides, 0.59% (11/1 874) Rhipicephalus pumilio and 0.43% (8/1 874) Rhipicephalus sanguineus. The 16S rRNA, 12S rRN, COI and ITS2 segments of ticks were amplified separately by PCR and sequenced. Phylogenetic analysis showed that R. microplus Y2 (sample number) was in the same branch with R. microplus from Inida (EU918188), Guizhou of China (KC503259), Malaysia (KM246873) and Guizhou of China (KC503274) through 16S rRNA, 12S rRNA, COI and ITS2 sequences analysis respectively, but was different R. microplus fomerly reported from Yunnan of China.R. haemaphysaloides Y5 was in the same branch with R. haemaphysaloides from Thailand (KC170743), Taiwan (DQ003005) and Hunan of China (KM083593) through 16s rRNA, 12S rRNA and COI sequences analysis respectively. R. pumilio Y6 and Y01 were in the same branch with R. pumilio from Australian (AF271282). It was concluded that R. microplus is the dominant tick species of domestic animals in Hengduan Mountains,the west of Yunnan Province. In addition,R. pumilio may be a new record in Yunnan.
亚红祥, 沈姝, 苏正元, 邓菲, 张云智. 滇西横断山区家畜体表蜱类调查及鉴定[J]. 中国人兽共患病学报, 2016, 32(10): 865-870.
YA Hong-xiang, SHEN Shu, SU Zheng-yuan, DENG Fei, ZHANG Yun-zhi. Tick species and genetic variants analysis of tick gene in Hengduan Mountains, west Yunnan Province, China. Chinese Journal of Zoonoses, 2016, 32(10): 865-870.
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