Construction and immunogenicity of a suicidal DNA vaccine with surface antigen SAG1 gene of Toxoplasma gondii
ZHENG Li-na1, 2, TAN Feng1, PAN Chang-wang1
1. Department of Parasitology, School of Basic Medical Sciences,Wenzhou Medical University, Wenzhou 325035, China; 2. School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou 325035, China
Abstract：We constructed a suicidal DNA vaccine pDREP-SAG1 encoding the major surface antigen SAG1 gene of Toxoplasma gondii and identify its immunogenicity. SAG1 gene (GenBank accession number: HM776940.1) fragment was amplified with PCR from genomic DNA of T. gondii RH strain. The PCR product was cloned into an alphavirus replicon-based vector pDREP-eGFP to replace the coding sequence of eGFP. Recombinant plasmid pDREP-SAG1 was confirmed by PCR, restriction enzymes digestion and sequencing. BALB/c mice were injected with the recombinant suicidal DNA vaccine pDREP-SAG1 intramuscularly into quadricep followed by immediate electroporation and boosted with the same condition once at 3-week intervals. Mice received injection with plasmid pDREP-eGFP were used as control. Sera collected from immunized mice were verified by Western blotting with Toxoplasma lysate antigen (TLA). Results showed that SAG1 gene of 1 011 bp was amplified from genomic DNA of T. gondii, and recombinant plasmid pDREP-SAG1 was successfully constructed. Sera collected from plasmid pDREP-SAG1 immunized mice could specifically recognize SAG1 antigen from TLA. The suicidal DNA vaccine pDREP-SAG1 was successfully constructed and showed its immunogenicity to induce specific antibody response in mice.
郑丽娜, 谭峰, 潘长旺. 弓形虫表面抗原SAG1基因自杀性DNA疫苗的构建及免疫原性检测[J]. 中国人兽共患病学报, 2016, 32(10): 885-888.
ZHENG Li-na, TAN Feng, PAN Chang-wang. Construction and immunogenicity of a suicidal DNA vaccine with surface antigen SAG1 gene of Toxoplasma gondii. Chinese Journal of Zoonoses, 2016, 32(10): 885-888.
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2016, Vol. 32 
