Abstract：This study aims to establish an ELISA method for detection of Campylobacter jejuni infection by using the B-cell antigen epitope polypeptide of OMP18 as the coating antigen. Using the B-cell epitope peptides with different concentrations (0.1,1 and 10 μg/mL) as the coating antigens, the rabbit anti-Campylobacter jejuni IgG antibody as the primary antibody and the HRP-labeled goat anti-rabbit IgG antibody as the secondary antibody, the researchers detect the levels of IgG antibody after different dilutions (1:10, 1:10 and 1:1 000) based on its original concentration of 1 mg/mL. The researchers also compare different immunological specificities of epitope peptides via using the serum from a healthy rabbit, the serums respectively from the rabbits infected with Campylobacter jejuni and Salmonella as the primary antibody as well as the HRP-labeled goat anti-rabbit IgG antibody(1:3 000) as the secondary antibody. The immunoreactivity of three B cell antigen epitope polypeptides of OMP18 significantly increase in the dilutions of 1:1 000,1:4 000 and 1:16 000 and 1:64 000,while it finds the most significant increase in a 1:1 000 dilution.Therefore, the B-cell epitope peptides of OMP18 indeed features an immunological specificity. And the study indicates that the ELISA is a desirable method capable of detecting Campylobacter jejuni infection by using OMP18 B cell antigen epitope polypeptide as the coating antigen with a high immune response and a specific immunity.
楼宏强, 高素华, 李旭升, 陈浩浩, 胡野. 基于OMP18的B细胞抗原表位的空肠弯曲菌ELISA检测方法的建立[J]. 中国人兽共患病学报, 2016, 32(10): 889-892.
LOU Hong-qiang, GAO Su-hua, LI Xu-sheng, CHEN Hao-hao, HU Ye. Establishment of ELISA method for detecting Campylobacter jejuni based on the B-cell antigen epitope of OMP18. Chinese Journal of Zoonoses, 2016, 32(10): 889-892.
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2016, Vol. 32 
