Abstract:Escherichia coli could cause diseases under certain conditions, O-antigens contribute to the virulence of E. coli. The chemical composition and structure of O-antigen on the surface of E. coli have high diversity, while specific O-antigen serotype have some connections with certain pathogenicity of E. coli. Thus, identification of O-antigen serotypes is important for epidemiological studies and control of diseases caused by pathogenic E. coli. The traditional serological typing method is time-consuming, expensive and inaccurate. With the development of science and technology, 196 O-antigen synthesis gene clusters of E. coli were sequenced, different O-antigen synthesis gene clusters were compared and analyzed, and molecular markers for specific DNA sequences were designed. PCR method was applied for serotyping O-antigens, including single PCR, multiple PCR, real-time PCR, DNA microarray and microbead-based suspension array. Besides, restriction of the amplified O-antigen gene cluster, microbead-based immunoassay and whole genome sequencing were also used for serotyping O-antigens. These methods would enrich the identification method of E. coli O-antigen serogroups and make up the deficiency of traditional serological typing method. This review focuses on the research progress about O-antigen synthesis gene cluster and O-antigen serotype identification methods.
刘璨颖, 张济培, 王丙云. 大肠杆菌O-抗原血清型鉴定研究进展[J]. 中国人兽共患病学报, 2016, 32(10): 928-933.
LIU Can-ying, ZHANG Ji-pei, WANG Bing-yun. Research progress on identification of Escherichia coli O-antigen serogroups. Chinese Journal of Zoonoses, 2016, 32(10): 928-933.
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