Abstract：To research the contamination status and distributive regulation of Legionella pneumophila in samples from spring water and analyze the main serotypes and genotypes of the pathogen, a stratified sampling method was adopted to collect water samples randomly from water tank, hot spring pool, shower room of hot spring recreations. Suspected strains were isolated from condensed water samples. Serum agglutination assay and real-time PCR technology were used to identify L. pneumophila. Pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) were used to describe the genetic polymorphism of the isolates. Overall, 53.85% of water samples showed Legionella-positive, and their concentrations ranged from 20 CFU/100 mL to 16 000 CFU/100 mL. The main serotypes were LP1 type and LP3 type and all strains carried dot gene. Among them, 40 isolates were analyzed by PFGE by which 10 distinct patterns were differentiated. They were also selected for SBT analysis and divided into 13 different sequence types in 3 main clonal groups belonging to one homomorphic type. Our results demonstrated high prevalence and genetic polymorphism of Legionella in hot spring recreations of Wenzhou. Hot spring area posed a potential threat to tourists. Spring water of these places should be intervened to prevent occurrence of Legionnaires? disease.
章乐怡, 李毅, 上官智慧, 吴跃进, 谢爱蓉, 王良怀, 陈文斌. 温泉水中嗜肺军团菌污染调查及分子分型研究[J]. 中国人兽共患病学报, 2016, 32(12): 1096-1101.
ZHANG Le-yi, Li Yi, SHANGGUAN Zhi-hui, WU Yue-jin, XIE Ai-rong, WANG Liang-huai, CHEN Wen-bin. Contamination status and molecular typing of Legionella pneumophila in spring environments. Chinese Journal of Zoonoses, 2016, 32(12): 1096-1101.
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2016, Vol. 32 
