Genetic analysis of Echinococcus granulosus strain in Liaoning Province, China
WANG Xiao-li1, LI Fei2, LI Jin-hui1, LIU Jing1, LI Ya-cai1, BAI Yang1, An chun-li3
1. Department of Endocrinology and Metabolism, Institute of Endocrinology, Key Laboratory ofEndocrine Diseases of Liaoning Province, the First Affiliated Hospital,China Medical University, Shenyang 110001, China;
2. Department of Gastroenterology and Endocrinology, Hospital of Tacheng,China Medical University,Tacheng 834799, China;
3. Department of Microbiology and Parasitology, College of Basic Medical Science,China Medical University, Shenyang, 110001,China
To analyze the genotype of Echinococcus granulosus found in Liaoning Province, hydatid cyst was taken from the patient?s liver, DNA of cyst was isolated and mitochondrial cox1 gene was sequenced substantially. Sequence results was analyzed by Blast and compared with similar strains in GenBank. Results showed that molecular analysis confirmed the echinococcosis by identical sequence of our strain with E. granulosus (AF297617) G1 genotype, with 0.1% difference. And the sequence of our strain was consistent with 25 other strains in different countries. In conclusion, genotyping of mitochondrial gene cox1 is a very useful tool for diagnosis of echinococcosis. We first reported a strain of E. granulosus in Liaoning Province, which provide novel molecular epidemiological data of this zoonosis.
王晓黎, 李飞, 李金慧, 刘静, 李亚彩, 白杨, 安春丽. 辽宁省朝阳市细粒棘球病的基因型分析[J]. 中国人兽共患病学报, 2017, 33(2): 110-114.
WANG Xiao-li, LI Fei, LI Jin-hui, LIU Jing, LI Ya-cai, BAI Yang, An chun-li. Genetic analysis of Echinococcus granulosus strain in Liaoning Province, China. Chinese Journal of Zoonoses, 2017, 33(2): 110-114.
[1] Pakala T, Molina M, Wu GY. Hepatic echinococcal cysts: A review[J]. J Clin Transl Hepatol, 2016, 4(1): 39-46.
[2] Rossi P, Tamarozzi F, Galati F, et al. The first meeting of the european register of cystic echinococcosis (ERCE)[J]. Parasit Vectors.2016, 28; 9:243.
[3] Cadavid Restrepo AM, Yang YR, McManus DP, et al. The landscape epidemiology of echinococcosis[J]. Infect Dis Poverty, 2016, 5: 13.
[4] van Cauteren D, Millon L, de Valk H, et al. Retrospective study of human cystic echinococcosis over the past decade in France, using a nationwide hospital medical information database[J]. Parasitol Res, 2016, 115(11): 4261-4265.
[5] Wen H, Tuerganaili A, Shao YM, et al. Achievements and challenges for echinococcosis control[J]. Chin J Parsitol Paraist Dis, 2015,33(6): 466-471. (in Chinese)
温浩,吐尔干戈力·阿吉,邵英梅等. 棘球蚴病防治成就及面临的挑战[J]. 中国寄生虫病防治杂志, 2015,33(6):466-471 .
[6] Ito A, Nakao M, Lavikainen A, et al. Cystic echinococcosis: Future perspectives of molecular epidemiology[J]. Acta Trop, 2016, pii: S0001-706X(16)30242-X.
[7] Romig T, Ebi D, Wassermann M. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato[J]. Vet Parasitol, 2015, 213(3/4): 76-84.
[8] McManus DP. Current status of the genetics and molecular taxonomy of Echinococcus species[J]. Parasitology, 2013, 140(13): 1617-1623.
[9] Alvarez Rojas CA, Romig T, Lightowlers MW. Echinococcus granulosus sensu lato genotypes infecting humans-review of current knowledge[J]. Int J Parasitol, 2014, 44(1): 9-18.
[10] Hu D, Song X, Wang N. et al. Molecular identification of Echinococcus granulosus on the Tibetan Plateau using mitochondrial DNA markers[J]. Genet Mol Res, 2015, 14(4): 13915-13923.
[11] Zhang T, Yang D, Zeng Z, et al. Genetic characterization of human-derived hydatid cysts of Echinococcus granulosussensu lato in Heilongjiang Province and the first report of G7 genotype of E.canadensis in humans in China[J]. PLoS One, 2014, 9(10): e109059.
[12] Yu JF, Li B, Liu XS, et al. Cloning and sequence analysis of the partial CO 1 gene with in mitochondria DNA of Echinococcus granulosus [J]. Chin J Zoonoses, 2015, 31(6): 532-536. (in Chinese)
于晶峰, 李滨, 刘晓松, 等.细粒棘球蚴线粒体 CO 1基因的克隆与序列分析[J]. 中国人兽共患病学报, 2015, 31(6): 532-536.
[13] Alvarez Rojas CA, Ebi D, Gauci CG, et al. Microdiversity of Echinococcus granulosus sensu stricto in Australia[J]. Parasitology, 2016, 143(8): 1026-1033.
[14] Ito A, Dorjsuren T, Davaasuren A, et al. Cystic echinococcoses in Mongolia: molecular identification, serology and risk factors[J]. PLoS Negl Trop Dis, 2014, 8(6): e2937.
[15] Konyaev SV, Yanagida T, Nakao M, et al. Genetic diversity of Echinococcus spp. in Russia[J]. Parasitology, 2013, 140(13): 1637-1647.
[16] Nakao M, Yanagida T, Konyaev S, et al. Mitochondrial phylogeny of the genus Echinococcus (Cestoda: Taeniidae) with emphasis on relationships among Echinococcus canadensis genotypes[J]. Parasitology, 2013, 140(13): 1625-1636.
[17] Yanagida T, Mohammadzadeh T, Kamhawi S, et al. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East[J]. Parasitol Int, 2012, 61(4): 599-603.
[18] Konyaev SV, Yanagida T, Ivanov MV, et al. The first report on cystic echinococcosis in a cat caused by Echinococcus granulosus sensu stricto (G1) [J]. J Helminthol, 2012, 86(4): 391-394.
[19] Pakala T, Molina M, Wu GY. Hepatic echinococcal cysts: A review[J]. J Clin Transl Hepatol, 2016, 4(1): 39-46.
[20] Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing[J]. Mol Biochem Parasitol, 1992, 54: 165-173.
[21] Bowles J, McManus D, Donald P. Molecular variation in Echinococcus [J]. Acta Trop, 1993, 53: 291-305.
[22] Nakao M, Lavikainen A, Yanagida T, et al. Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae) [J]. Int J Parasitol, 2013, 43(12/13): 1017-1029.
[23] Spotin A, Mahami-Oskouei M, Harandi MF, et al. Genetic variability of Echinococcus granulosus complex in various geographical populations of Iran inferred by mitochondrial DNA sequences[J]. Acta Trop, 2016, pii: S0001-706X(16)30092-4.
[24] Yang YR, Rosenzvit MC, Zhang LH, et al. Molecular study of Echinococcus in west-central China[J]. Parasitology, 2005, 131(Pt 4): 547-555.
[25] Wang N, Gu XB, Wang T, et al. Genetic variability of Echinococcus granulosus determined by the mitochondrial cytochrognme coxidase subunit 1 gene in the Tibet plateau of China[J]. Acta Veterinaria et Zootechnica Sinica, 2015, 46(3): 453-460. (in Chinese)
王凝, 古小彬, 汪涛, 等. 基于 COX 1基因对中国青藏高原地区细粒棘球绦虫遗传多态性的研究[J]. 畜牧兽医学报,2015,46(3):453-460.
[26] Yang JK, Jia WZ, Jing T, et al. Analysis of E. granulosus gene variation in three provinces in china[J]. Chin J Vet Sci Technol, 2004, 34(9): 12-16. (in Chinese)
杨俊克,贾万忠,景涛,等.我国三省区细粒棘球绦虫基因的变异分析[J].中国兽医科技,2004,34(9):12-16.
[27] Cao DP, Fan HN, Wu DF, et al. Phylogeny of Qinghai plateau Echinococcus granulosus isolates inferred by cytochromeoxidase 1 gene sequences[J]. Chin J Zoonoses, 2014, 30(10): 1048-1051. (in Chinese)
曹得萍,樊海宁,毋德芳,等.利用细胞色素氧化酶亚单位Ⅰ分析青海藏区细粒棘球绦虫系统发育学[J]. 中国人兽共患病学报[J], 2014,30(10):1048-1051.
