Identification of non-tuberculosis mycobacteria species of clinical isolates from patients clinically diagnosed with tuberculosis in Fujian Province, China
LIU Hai-can1, HUANG Ming-xiang2, JIANG Yi1, LIAN Lu-lu1, 3, ZHAO Xiu-qin1, ZHANG Li-shui2, WU Yi-mou3, WAN Kang-lin1, 3
1. State Key Laboratory for Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2. Fuzhou Pulmonary Hospital,Fuzhou 350008, China; 3. Pathogenic Biology Institute, University of South China, Hengyang 421001, China
Abstract：Some species of non-tuberculosis mycobacteria (NTM) are important causes of human disease and infections. The purpose of this study was to describe the NTM species of clinical isolates from patients with tuberculosis in Fujian, China. The clinical NTM strains isolated from the patients clinically diagnosed with tuberculosis collected from 2005 to 2011 in Fuzhou Pulmonary Hospital, Fujian Province, and were identified to species level using conventional and molecular methods including multi-loci PCR, PRA-hsp65 and PRA-rpoB and sequencing for hsp65, rpoB, 16S rRNA and ITS. A total of 450 pre-identified NTM isolates were identified as 45 (10.00%) Mycobacterium tuberculosis strains and 405 (90.00%) NTM strains, which including 23 different NTM species and a Gordonia bronchialis isolates. There were at least 23 NTM species as potential pathogens were found to cause pulmonary infections in human beings, and 6 NTM species and Gordonia bronchialis were firstly identified in patients with a clinical diagnosis of tuberculosis (TB) in Fujian Province. NTM species identification is very important for distinguishing between TB and NTM pulmonary diseases. And combination of several methods can increase identification accuracy and efficacy.
刘海灿, 黄明翔, 蒋毅, 连璐璐, 赵秀芹, 张丽水, 吴移谋, 万康林. 福建省肺非结核分枝杆菌临床分离株的菌种鉴定[J]. 中国人兽共患病学报, 2017, 33(5): 389-397.
LIU Hai-can, HUANG Ming-xiang, JIANG Yi, LIAN Lu-lu, ZHAO Xiu-qin, ZHANG Li-shui, WU Yi-mou, WAN Kang-lin. Identification of non-tuberculosis mycobacteria species of clinical isolates from patients clinically diagnosed with tuberculosis in Fujian Province, China. Chinese Journal of Zoonoses, 2017, 33(5): 389-397.
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