Abstract:We isolated and identified the bacterial pathogen in a pyogenic balanoposthitis patient and investigated the drug resistance and its mechanism of the isolate. Urethral secretions and balanus pustule liquids were collected for microscopic examination after Gram-staining and detection of mycoplasma using Mycoplasma IST 2 kit. The two samples were inoculated on Columbia blood plate, N. gonorrhoeae selective plate and chromID Candida plate for isolation. The obtained colonies were identified by VITEK 2-compact automatic bacterial detection and analysis system. Moreover, PCR was performed to detect 16S rRNA gene of N. gonorrhoeae in the samples and colonies. KB method was applied for detecting susceptibility of five common antibiotics against the isolate. The β-lactamase and extended spectrum β-lactamase confirmatory tests were used to investigate the enzyme production of the isolate as well as drug resistance-associated tetM, TEM, mefA and ermF genes in the isolate were detected by PCR. Results showed that all the clinic samples showed negative for mycoplasma. All the isolating cultivation results of urethral secretions were negative while the balanus pustule liquids provided positive isolating cultivation in the blood and selective plates. The VITEK 2-compact system and 16S rRNA-PCR revealed that the isolated strain belongs to N. gonorrhoeae. The isolate can produce β-lactamases and resist to penicillin G, ciprofloxacin and tetracycline. The tetM, TEM, mefA and ermF genes could be found in the isolate’s genome. The patient’s balanoposthitis is caused by infection of N. gonorrhoeae. The multidrug resistance of Neisseria gonorrhoeae isolate is closely associated with its carried resistant genes.
葛玉梅, 胡庆丰, 朱永泽, 周永列, 吕火烊. 龟头包皮炎患者淋病奈瑟菌分离与鉴定及其耐药机制研究[J]. 中国人兽共患病学报, 2017, 33(5): 432-435.
GE Yu-mei, HU Qing-feng, ZHU Yong-ze, ZHOU Yong-lie, LYU Huo-yang. Isolation and identification of Neisseria gonorrhoeae strain from a balanoposthitis patient and drug resistance mechanism of the isolate. Chinese Journal of Zoonoses, 2017, 33(5): 432-435.
[1] Sunita G, Sarika A, Shreekant V, et al. Rice and Sanjay Ram Properdin is critical for antibody-dependent bactericidal activity against Neisseria gonorrhoeae that recruit C4b-binding protein[J]. J Immunol,2012,188(7): 3416-3425. doi:10.4049/jimmunol. 1102746 [2] Gong XD, Yue XL, Jiang N, et al. Epidemiological characteristics and trends of gonorrhea in China from 2000 to 2014[J]. Chin J Dermatol, 2015, 48(5): 301-306. doi:10.3760/cma.j. issn.0412-4030.2015.05.002 [3] Yan J. Medical microbiology[M]. 3rd Edition, Beijing: The High Education Publication House, China, 2016: 25-26, 82-84. (in Chinese) [4] Dong HL, Guo YY, Mao JF, et al. Analysis on the results of main sexually transmitted diseases tested in 6 682 male cases suspected with genitourinary infection[J]. Chin J Zoonoses, 2014,30(1): 102-105. doi:10.3969/cjz.j.issn.1002-2694.2014.01.022 [5] David J, Farrel L. Evaluation of amplicor Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR[J]. J Clin Microbiol,1999,37(2): 386-390. DOI: 0095-1137/99 /04.0010 [6] Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing[S]. Twenty-fourth Informational Supplement. CLSI document M100-S24. Wayne: Clinical and Laboratory Standards Institute. 2014. [7] Laura M, Valerie C, Zaelle D, et al. First Neisseria gonorrhoeae genotyping analysis in France: identification of a strain cluster with reduced susceptibility to ceftriaxone[J]. J Clin Microbiol,2009,47(11): 3540-3545. doi:10.1128/JCM.01236-09 [8] Joseph RD, Lalitagauri MD, Douglas JB, et al. Evolution and dissemination of extended spectrum β-lactamase-producing Klebsiella pneumoniae : Epidemiology and molecular report from the SENTRY Antimicrobial Surveillance Program (1997-2003)[J]. Diagn Microbiol Infect Dis,2005,51(1): 1-7. doi:10.1016/j.diagmicrobio.2004.08.001 [9] Chunxin W, Peiquan C, Dong C, et al. A Pseudomonas aeruginosa isolate producing the GES-5 extended-spectrum β-lactamase[J]. J Antimicrob Chemoth, 2006, 57(6): 1261-1262. doi:10.1093/jac/dkl116 [10] Li GM, Chen Qun, Chen JJ, et al. Resistance genes of multi-drug resistance strains of Neisseria gonorrhoeae [J].Chin J Nosocomiol Vol, 2005, 15(8): 852-854. doi:10.3321/j.issn:1005-4529.2005.08.004 [11] Ros CTD, Schmitt CDS. Global epidemiology of sexually transmitted diseases[J]. Lancet, 2008, 10(1): 110-114. doi:10.1111/j.1745-7262.2008.00367 [12] Johnson MB, Criss AK. Resistance of Neisseria gonorrhoeae to neutrophils[J]. Front Microbiol,2011,2(1): 77-77. doi:10.3389/fmicb.2011.00077 [13] Yin F, Feng Z, Li X. Spatial analysis of county-based gonorrhoea incidence in mainland China from 2004 to 2009[J]. Sex Health,2012,9(3): 227-232. doi:10.1071/SH11052 [14] Unemo M, Nicholas RA. Emergence of multi-drug resistant, extensively drug-resistant and untreatable gonorrhea[J]. Future Microbiol, 2012, 7(12): 1401-1422. doi:10.2217/ fmb.12.117 [15] Unemo M, Shafer WM. Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution and future[J]. Clin Microbiol Rev, 2014, 27(3): 587-613. doi:10.1128/CMR.00010-14 [16] Luna VA, Cousin S, Whittington WLH, et al. Identification of the conjugative mef gene in clinical Acinetobacter junii and Neisseria gonorrhoeae isolates[J]. Antimicrob Agents CH, 2000,44(9): 2503-2506. doi:10.1128/AAC.44.9.2503-2506.2000 [17] Flrez AB, Ammor MS, Martín PL, et al. Molecular analysis of tet(W) gene-mediated tetracycline resistance in dominant intestinal bifidobacterium species from healthy humans[J]. Appl Environ Microb, 2006, 72(11): 7377-7379. doi:10.1128/AEM.00486-06 [18] Li YW, Shi ZQ, Zhao PL, et al. The research on plasmid profiles of TEM-1 encoding gene in penicillinase-producing Neisseria gonorrhoeae and tetM gene in high level tetracycline-resistant Neisseria gonorrhoeae in Fosha[J]. Chin J Antibiot,2014, 39(3): 236-239. doi:10.3969/j.issn.1001-8689.2014.03.016