Molecular characteristics of the full-length genome of dengue serotype 1 virus strains isolated from dengue fever cases in Sino-Myanmar border region in Yunnan Province, China
HU Ting-song1, ZHANG Hai-lin1, LIU Yong-hua2, XU Song-miao1, YIN Xiao-xiong2, LI Hua-chang3, DENG Bo1, HUANG Ying4, ZHANG Fu-qiang1, FAN Quan-shui1
1. Center for Disease Control and Prevention, Chengdu Military Region, Kunming 650118, China;
2. Ruili Center for Disease Control and Prevention, Ruili 678600, China;
3. Lincang Prefecture Center for Disease Control and Prevention, Lincang 677000, China;
4. The Third People’s Hospital of Kunming, Kunming 650041, China
We investigated the molecular characteristics of the full-length genome of 14 dengue serotype 1 virus (DENV-1) strains isolated in Sino-Myanmar border region in Yunnan Province, China during 2013-2015. Isolation of dengue virus was using C6/36 cell culture method. Viral RNA was extracted from virus isolates, and then the full-length genome was amplified by RT-PCR. The homology and phylogenetic analysis was made on the nucleotide and deduced amino acid sequences by bioinformatics software including ClastalX1.83 and MEGA6 etc. Results showed that fourteen strains of DENV-1 isolated from dengue fever cases, of these, 9 strains from Ruili City of Dehong Prefecture, 3 from Lincang Prefecture, 2 from Kunming City. RT-PCR and sequencing indicated that the full-length genes (10 735 nt) of 14 DENV-1 strains were obtained, and their open reading frame (95-10 271) were coded 3 392 amino acid residues. The genotypes of DENV-1 were revealed by homology and phylogenetic analysis based on structural and non-structural proteins. Thirteen were genotype I (G-I) (7 from indigenous cases in Ruili and Lincang and 6 from imported case from Myanmar to Ruili, Lincang and Kunming), and 1 G-III from imported case from India to Kunming. The phylogenic analysis indicated that the 13 isolates from Yunnan divided into 2 phylogenic subgroups, and they had a closer genetic relationship with the strains isolated from Southeast Asia. The gene sequences of the 13 G-I strains have been acquired, the rate of their nucleotide homology and amino acid homology were 97.02%-100% and 98.78%-100% respectively. Compared with 6 strains from Southeast Asia,nucleotide homology and amino acid homology were 96.53%-99.53% and 97.33%-100% respectively. Compared with prototype strain (US_Hawaii) of DENV-1,nucleotide homology and amino acid homology were 93.76%-94.45% and 95.86%-96.91% respectively.Compared with US_Hawaii strain, there were 44 and 150 different sites in amino acid of structural and non-structural proteins, respectively. The G-1 of DENV-1 have been popular in Sino-Myanmar border region in Yunnan, 2013-2015. They have genetic diversity but multiple transmission sources were from Myanmar, and should strengthen control cross-border spread of dengue fever in this region. It is necessary to further study that change of the amino acid sites of Yunnan strains of DENV-1 is related to its antigenicity and pathogenicity.
胡挺松, 张海林, 刘永华, 徐松淼, 李华昌, 邓波, 尹小雄, 黄瑛, 张富强, 范泉水. 云南中缅边境登革1型病毒全基因组序列特征研究[J]. 中国人兽共患病学报, 2017, 33(6): 473-480.
HU Ting-song, ZHANG Hai-lin, LIU Yong-hua, XU Song-miao, YIN Xiao-xiong, LI Hua-chang, DENG Bo, HUANG Ying, ZHANG Fu-qiang, FAN Quan-shui. Molecular characteristics of the full-length genome of dengue serotype 1 virus strains isolated from dengue fever cases in Sino-Myanmar border region in Yunnan Province, China. Chinese Journal of Zoonoses, 2017, 33(6): 473-480.
[1] WHO and the Special Programme for Research and Training in Tropical Diseases.Dengue guidelines for diagnosis, treatment, prevention and control: new edition[R].Geneva: WHO, 2009: 3-16.
[2] Bureau of Disease Control and Prevention, Health Department of the People’s Republic of China.Handbook of prevention and control for dengue fever[M].2nd ed.Beijing: People’s Health Press, 2008: 13-23.(in Chinese)
卫生部疾病预防控制局.登革热防治手册[M].2版.北京: 人民卫生出版社,2008: 13-23.
[3] Kraemer MU, Sinka ME, Duda KA, et al: The global distribution of the arbovirus vectors Aedes aegypti and Ae.albopictus [J].eLife, 2015, 4:e08347.doi:10.7554/eLife.08347.001
[4] Sun J, Wu D, Zhou H, et al.The epidemiological characteristics and genetic diversity of dengue virus during the third largest historical outbreak of dengue in Guangdong, China, in 2014[J].J Infect, 2016, 72(1): 80-90.doi:10.1016/j.jinf.2015.10.007
[5] Wang JZ, Chen HB, Zhang YJ, et al.Sequence for E genes of autochthonous dengue virus isolates from Nanping, Fujian province, 2014[J].Chin J Zoonoses, 2016, 32(3): 281-285.doi:10.3969/j.issn.1002-2694.2016.03.014 (in Chinese)
王金章,陈宏彬,张拥军,等.2014年福建省南平市登革病毒E基因序列分析[J].中国人兽共患病学报,2016,32(3):281-285.doi:10.3969/j.issn.1002-2694.2016.03.014
[6] Sui HT, Ma XJ, Yang YC, et al.Dengue fever outbreak in a district of Fuzhou, Fujian, China, 2015[J].Chin J Zoonoses, 2016, 32(12): 1131-1134.doi:10.3969/j.issn.1002-2694.2016.012.017 (in Chinese)
随海田,马晓军,杨益昌,等.福建省福州市鼓楼区一起登革热暴发疫情现场调查报告[J].中国人兽共患病学报,2016,32(12):1131-1134.doi:10.3969/j.issn.1002-2694.2016.012.017
[7] Zhang HL, Fu SH, Deng Z, et al.An outbreak of imported dengue fever from Myanmar to the border of China, with its viral molecular epidemiological features[J].Chin J Epidemiol, 2013, 34(5): 428-432.doi:10.3760/cma.j.issn.0254-6450.2013.05.004 (in Chinese)
张海林,付士红,邓掌,等.云南中缅边境一起输入性登革热暴发的分子流行病学研究[J].中华流行病学杂志,2013,34(5):428-432.doi:10.3760/cma.j.issn.0254-6450.2013.05.004
[8] Jiang JY, Guo XF, Tang YR, et al.Surveillance and control of imported dengue cases in Yunnan from 2004 to 2014[J].Chin J Vector Biol Ctrl, 2016, 27(1): 5-8.doi:10.11853/j.issn.1003.4692.2016.01.002 (in Chinese)
姜进勇,郭晓芳,唐烨榕,等.云南省2004-2014年输入性登革热病例监测与防控对策分析[J].中国媒介生物学及控制杂志,2016,27(1):5-8.doi:10.11853/j.issn.1003.4692.2016.01.002
[9] Feng Y, Liu YH, Yin ZL, et al.Molecular epidemiologic analysis of an outbreak of dengue fever in 2013 at Ruili city, Yunnan Province of China[J].Chin J Viral Dis, 2014, 4(4): 306-311.(in Chinese)
冯云, 刘永华, 尹正留, 等.云南省瑞丽市2013年登革热暴发的分子流行病学研究[J].中国病毒病杂志, 2014, 4(4):306-311.
[10] Liu YH, Feng Y, Yin XX, et al.Molecular epidemiology of dengue virus types 1 and 2 in Ruili, Yunnan, 2014[J].Dis Surveill, 2016, 31(1): 8-13.doi:10.3784/j.issn.1003-9961.2016.01.004 (in Chinese)
刘永华,冯云,尹小雄,等.2014年云南省瑞丽市登革1和2型病毒流行的分子流行病学研究[J].疾病监测,2016, 31(1):8-13.doi:10.3784/j.issn.1003-9961.2016.01.004
[11] Li HC, Pan H, Feng Y, et al.Epidemiological survey of an outbreak of dengue fever in Lincang, Yunnan, 2015[J].Dis Surveill, 2016, 31(7): 561-565.doi:10.3784/j.issn.1003-9961.2016.07.007 (in Chinese)
李华昌,潘虹,冯云,等.2015年云南省临沧市登革热暴发的流行病学调查[J].疾病监测,2016, 31(7):561-565.doi:10.3784/j.issn.1003-9961.2016.07.007
[12] Tamura K, Stecher G, Peterson D, et al.MEGA6: Molecular Evolutionary Genetics Analysis version 6.0[J].Mol Biol Evolut, 2013, 30(12): 2725-2729.
[13] Rico-Hesse R.Molecular evolution and distribution of dengue viruses type 1 and 2 in nature[J].Virology, 1990, 174(2): 479-493.
[14] Villabona-Arenas CJ, Zanotto PMd.Worldwide spread of dengue virus type 1[J].PLoS One, 2013, 8(5): e62649.doi:10.1371/journal.pone.0062649
[15] Fahri S, Yohan B, Trimarsanto H, et a1.Molecular surveillance of dengue in Semarang, Indonesia revealed the circulation of an old genotype of dengue virus serotype-1[J].PLoS Negl Trop Dis, 2013, 7(8): e2354.doi:10.1371/journal.pntd.0002354
[16] Thu HM, Lowry K, Myint TT, et al.Myanmar dengue outbreak associated with displacement of serotypes 2, 3, and 4 by dengue 1[J].Emerg Inf Dis, 2004, 10(4): 593-597.
[17] Dubot-PA, Vongphrachanh P, Denny J, et al.An epidemic of dengue-1 in a Remote Village in Rural Laos[J].PLoS Negl Trop Dis, 2013, 7(8): e2360.doi:10.1371/journal.pntd.0002360
[18] Kutsuna S, Kato Y, Moi ML, et al.Autochthonous dengue fever, Tokyo, Japan, 2014[J].Emerg Infect Dis, 2015, 21(3): 517-520.doi:10.3201/eid2103.141662
[19] Bray M, Men R, Tokimatsu I, et al.Genetic determinants responsible for acquisition of dengue type 2 virus mouse neurovirulence[J].J Virol, 1998, 72(2): 1647-1651.
[20] Rey FA, Heinz FX, Mandl C, et al.The envelope glycoprotein from tick-borne encephalitis virus at 2 A resolution[J].Nature, 1995, 375(6529): 291-298.doi:10.1038/375291a0
2017, Vol. 33 
