Detecting Salmonella with matrix assisted laser desorption ionization time of flight mass spectrometry
ZHAO Jian-hong1, GAO Yan1, LI Shu-ming1, ZHANG Shi-yao1, WANG Heng-wei1, GU Lin1, HAN Qing-hua1, JIAO Yang1, CHEN Qian2
1.Chaoyang Center for Disease Control and Prevention, Beijing 100021, China; 2.Beijing Center for Disease Control and Prevention,Beijing Center for Preventive Medical Research,Beijing 100013,China
Abstract：In order to optimize the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) method for Salmonella identification, study the target board pollution situation of different pretreatment methods, and establish a safe and effective method for detection of Salmonella, we applied MALDI-TOF-MS technique to detect 4 standard strains of Salmonella under different culture conditions and sample preparation method, and established optimization program. MALDI target board was detected under different preparation treatments. The optimization method was used to detect 33 strains Salmonella which isolated from diarrhea patients’ stool. Identification results were compared with serological results. The study found that MALDI-TOF-MS method could accurate identify of Salmonella in different pretreatment methods and culture medium. Thirty-three strains of Salmonella identified by MALDI-TOF-MS method were all accurate appraisal in genus level, 19 strains of them were completely consistent with the serotyping identification results, 14 strains of them were not consistent with the serotyping identification results. There was no bacteria growth on the target board in different pretreatment methods. MALDI-TOF-MS method can in a fast,convenient,safe and accurate way identify Salmonella, and it can become an effective means for identification of Salmonella.
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2017, Vol. 33 
