Gene chip technology for detecting mycobacteria and Mycobacterium tuberculosis resistance of isoniazid and rifampin in the application of tuberculous empyema
LIN Xiu-hua1, LAI Guo-xiang1, CHEN Yu-yan3, ZHENG Dan4, ZHOU Yin-fa5, CHEN Xiao-hong2
1. Department of Respiratory and Critical Care Medicine, Fuzhou General Hospital affiliated to Fujian Medical university,Fuzhou,350025,China; 2.Department of Tuberculosis, Fuzhou pulmonary hospital affiliated to Fujian Medical university, Fuzhou 350008,China; 3. The third people's hospital affiliated to Fujian university of traditional Chinese medicine, Fuzhou 350108,China; 4.Department of Clinical laboratory,Fuzhou pulmonary hospital affiliated to Fujian Medical university, Fuzhou 350008,China; 5.Fujian provincial center for disease control and prevention,Fuzhou 350008,China
Abstract：To evaluate the clinical value of gene chip technology (GCT) in detecting the mycoba-cteria, isoniazid and rifampin resistance of patients diagnosed tuberculous empyema.The 182 patients who met the inclusion criteria were enrolled to this study from January 2011 to December 2015, whose pus mycobacterial species were detected by GCT and MGIT, the simultaneous and sensitivity of them were compared. Meanwhile, 36 patients diagnosed tuberculous empyema were selected to detect isoniazid and rifampin resistance.The simultaneous and sensitivity of GCT were evaluated base on the standard of MGIT. The 135 patients were diagnosed by tuberculous empyema. The specificity of GCT was same to MGIT (95.7%), the the sensitivity was 48.9%(66/135)in GCT,26.7% in MGIT, there was significant difference between them (χ2=80.5,P<0.05).The sensitivity, specificity and coincidence rate of GCT in rifampin resistance were 100%,the sensitivity, specificityand coincidence rate in INH were 50.0%(1/2),97.1%(33/34) and 94.4%. Gene chip technology for detection of mycobacteria has high sensitivity and specificity, which can identify non-tuberculous mycobacteria quickly. And it can also effectively detect the resistance of Mycobacterium tuberculosis to isoniazid and rifampicin.It has important significance in early diagnosis and treatment of tuberculous empyema.
林秀华, 赖国祥, 陈雨燕, 郑丹, 周银发, 陈晓红. 基因芯片技术检测分枝杆菌和异烟肼、利福平耐药性在结核性脓胸诊断中的应用[J]. 中国人兽共患病学报, 2017, 33(8): 720-723.
LIN Xiu-hua, LAI Guo-xiang, CHEN Yu-yan, ZHENG Dan, ZHOU Yin-fa, CHEN Xiao-hong. Gene chip technology for detecting mycobacteria and Mycobacterium tuberculosis resistance of isoniazid and rifampin in the application of tuberculous empyema. Chinese Journal of Zoonoses, 2017, 33(8): 720-723.
[1] Yan BY,Duanmu HJ.Tuberculosis[M].Beijing:Beijing Publishing House,2003:576-587.(in Chinese) 严碧涯,端木宏谨.结核病学[M].北京:北京出版社,2003:576-587. [2] Bravo LT, Tuohy MJ, Ang C, et al. Pyrosequencing for rapid detection of Mycobacterium tuberculosis resistance to rifampin, isoniazid, and fluoroquinolones[J]. Clin Microbiol, 2009, 47(12): 3985-3990.doi:10.1128/JCM.01229-09 [3] Pietzka AT, Indra A, Stoger A, et al. Rapid identification of multigrug-resistant Mycobacterium tuberculosis isolate by rpoB gene scanning using high-resolution melting curve PCR analysis[J]. J Antimicrob Chemother, 2009, 63: 1121-1127.doi:10.1093/jac/dkp124 [4] Ahmad S, Mokaddas E. Recent advances in the diagnosis and treatment of multidrug-resistant tuberculosis[J]. Respir Med, 2009, 103(12): 1777-1790.doi:10.1016/j.rmed.2009.07.010 [5] Zhu L, Jiang G, Wang S, et al. Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum[J]. J Clin Microbiol, 2010, 48(10): 3654-3660.doi:10.1128/JCM.00158-10 [6] Huang MX,Wang L,Zhang LS,et al.Rapid identification of Mycobacterium species by DNA chip[J].Chin J Zoonoses,2010,26(6):555-557.(in Chinese) 黄明翔,王琳,张丽水,等.DNA芯片鉴定分枝杆菌的研究[J].中国人兽共患病学报,2010,26(6):555-557. [7] Gingeras TR, Ghandour G, Wang E, et al. Simultaneous genotyping and species identification using hybridization pattern recognition analysis of generic Mycobacterium DNA arrays[J]. Genome Res, 1998, 8(5): 435-448.doi:10.1101/gr.8.5.435 [8] Zhao LS,Dai D,Chen X,et al.Diagnostic value of gene chip in identification of mycobacterium and detection of drug ‐resistant genes[J].Lab Med Clin,2014,11 (12):1595-1598.(in Chinese) 赵连爽,代娣,陈昕,等.基因芯片在分枝杆菌菌种鉴定及结核耐药基因检测的诊断价值[J].检验医学与临床,2014,11(12):1595-1598. [9] Zhang LQ,Wang YX,Yao CY,et al.High specificity gene chip methods for detection of Mycobacterium tuberculosis :a clinical evaluation[J]. Chin J Nosocomiol,2010,20 (11) :1505-1508.(in Chinese) 张立群,王云霞,姚春艳,等.高特异性基因芯片法检测结核分枝杆菌的临床应用评价[J].中华医院感染学杂志,2010,20(11):1505-1508. [10] Jia FZ,Li LJ.Infectious disease[M].Nanjing:Jiangsu Science and Technology Press, 2010: 545-550.(in Chinese) 贾辅忠,李兰娟.感染病学[M].南京:江苏科学技术出版社,2010:545-550. [11] Tang SJ,Gao W.Clinical tuberculosis[M].Beijing:People’s Health Publishing House,2011:700-709.(in Chinese) 唐神结,高文.临床结核病学[M].北京:人民卫生出版社,2011:700-709. [12] Taiwo B, Glassroth J. Nontuberculous mycobacterial lung diseases[J]. Infect Dis Clin North Am, 2010, 24: 769-789.doi:10.1016/j.idc.2010.04.008 [13] Ma Y.Focus on diagnosis and treatment of non-tuberculous mycobacteria lung disease[J].Chin J Tuberc Respir Dis,2011,34:566-568.(in Chinese) 马玙.关注非结核分枝杆菌肺病的诊断与治疗[J].中华结核和呼吸杂志,2011,34:566-568. [14] Ma Y,Wang J.Pay attention to the identification of non-tuberculosis mycobacteria and pulmonary tuberculosis[J]. J Clin Pulm Med,20l0,15:301-302. [15] Deng YH,Xiang YG,Ma XH,et al.Identification of drug-resistance gene type in Mycobacterium tuberculosis by gene chip in Hunan province[J].Int J Lab Med,2015,36(22):3223-3226.(in Chinese) 邓叶华,向延根,马小华,等.基因芯片法用于检测长沙地区结核分枝杆菌耐药性研究[J].国际检验医学杂志,2015,36(22):3223-3226. [16] Ou WZ,Chen ZH,Chen J,et al.Determination of resistance genes Kat G and inh A in Mycobacterium tuberculosis isolates from Guizhou Province by gene chip[J]. Chin J Zoonoses,2015,31(7):655-658.doi:10.3969/cjz.j.issn.1002-2694.2015.07.013(in Chinese) 欧维正,陈峥宏,陈静,等. 基因芯片技术检测贵州省结核分枝杆菌耐药基因 KatG 和 inhA [J].中国人兽共患病学报,2015,31(7):655-658. [17] Ran B,Cai L.Diagnostic accuracy of gene chip in identifying rifampicin resistance Mycobacterium tuberculosis :a Meta-analysis[J].Chin J Antituberc,2015,37(1):56-65. [18] Zhang CL,Zhao LN,Ren H.Application of gene chip technology to detect the four kinds of TB research of drug sensitive test[J].Harbin Medical J,2015,35(3):182-184.
2017, Vol. 33 
