Distribution and typing of animal-derived Salmonella with MLST and serotype
ZHAO Cui1, ZHANG Qing2, GUO Shu-yuan3, LI Lu-lu2, LIU Yu-jie4, WANG Yong-ming5, CHANG Wei-shan3, LIU Yu-qing2
1. Tai’an City Animal Health Supervision Center in Shandong Province, Tai’an 271000, China; 2. Animal Husbandry and Veterinary Institute, Shandong Academy of Agricultural Science, Jinan 250100, China; 3. Animal Science Academy of Shandong Agricultural University, Tai’an 271018, China; 4. Animal Epidemic Prevention and Control Center in Rizhao City, Rizhao 276800, China; 5. Shandong Hua Hong Biological Products Co., Ltd., Binzhou 256600, China
Abstract：To compare the discriminatory ability of multilocus sequence typing and serotype of animal-derived Salmonella and find its distribution in Shandong Province, 78 chicken-origin, 56 duck-origin and 20 swine-origin Salmonella were separated from some regions of Shandong Province. Seven conserve sequences of Salmonella were PCR-amplified for MLST and slide agglutination test for serotyping. Results showed that by serotyping, 6 serotypes were identified from chicken-origin Salmonella, including 88.5% S. enteritidis, 5.1% S. indiana, 2.6% S. thompson, 1.3% S. typhimurium, 1.3% S. senftenberg, 1.3% S. agama. Two serotypes were identified from duck-origin Salmonella, including 67.9% S. enteritidis, 32.1% S. typhimurium. Three serotypes were identified from swine-origin Salmonella, including 65% S. typhimurium, 20% S. derby, and 15% S. enteritidis. By MLST typing, seven ST types were identified from chicken-origin Salmonella: ST11, ST19, ST26, ST128, ST14, ST17 and New1. Three ST types were identified form duck-origin Salmonella: ST11, ST19 and New2.Three ST types were identified from swine-origin Salmonella: ST34, ST40 and ST3007. Overall, the types identified with two methods were closed, so MLST and serotype have similar discriminatory ability.
赵翠, 张庆, 郭树源, 李璐璐, 刘玉洁, 王永明, 常维山, 刘玉庆. 山东省动物源沙门氏菌MLST和血清分型与分布研究[J]. 中国人兽共患病学报, 2017, 33(9): 793-799.
ZHAO Cui, ZHANG Qing, GUO Shu-yuan, LI Lu-lu, LIU Yu-jie, WANG Yong-ming, CHANG Wei-shan, LIU Yu-qing. Distribution and typing of animal-derived Salmonella with MLST and serotype. Chinese Journal of Zoonoses, 2017, 33(9): 793-799.
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