1. Fujian Center for Disease Control and Prevention, Fuzhou 350001, China; 2. Fujian Key Laboratory of Zoonoses, Fuzhou 350001, China; 3. Public Health School of Fujian Medical University, Fuzhou 350001, China
Abstract：We delineated the molecular characteristic of recombinant strain GII.P16/GII.2 of norovirus associated with acute viral gastroenteritis outbreaks in Fujian Province in winter of 2016. Norovirus were detected in specimens of patients collected from the gastroenteritis outbreaks by real-time reverse transcription-PCR and reverse transcription-PCR (RT-PCR). The PCR products of the positive samples were purified, and partial RNA dependent RNA polymerase (RdRp) gene and partial capsid gene were sequenced. The sequences were analyzed using bioinformatics software and online database,and phylogenetic tree were also constructed. Norovirus were detected in all 18 stools. Analysis of 9 positive sequences indicated an emergence of norovirus GII. P16/ GII.2 and confirmed being the cause of gastroenteritis outbreaks. All the strains shared homology of 98% with strains of Kawasaki 194 of Japan detected in 2016 and 97.7%-98.8% with IPH2161-08VG06 of Belgium detected in 2008, RdRp and capsid separately. These outbreak strains showed some degree of differences from the predominant strain, 2012 Sydney GII.4 variant. This is the first time to have found norovirus GII.P16/ GII.2 causing viral gastroenteritis outbreaks in Fujian. More in-depth analysis of the Norovirus GII.P16/ GII.2 could be useful to optimize preventative strategies and develop new and more effective therapeutic measure.
[1] Wu BS, Xie JF, Shen XN, et al. Epidemiological research of noroviruses causing Viral gastroenteritis outbreaks in Fujian[J]. Chin J Epidemiol, 2008, 29(4): 382. (in Chinese) 吴冰珊,谢剑锋,沈晓娜.福建省诺如病毒腹泻暴发的流行病学研究. [J].中华流行病学杂志,2008,29(4):382. [2] Honma S, Nakata S, Kinoshita-Numata K, et al. Evaluation of nine sets of PCR primers in the RNA dependent RNA polymerase region for detection and differentiation of members of the family Caliciviridae, Norwalk virus and Sapporo virus[J]. Microbiol Immunol, 2000, 44: 411-419. [3] Vinjé J,Vennema H,Maunula L,et al. International collaborative study to compare reverse transcriptase PCR assays for detection and genotyping of noroviruses[J]. J Clin Microbiol,2003, 41(4): 1423-1433. [4] Kroneman A, Vennema H, Deforche K, et al. An automated genotyping tool for enteroviruses and noroviruses[J]. J Clin Virol, 2011, 51(2):121-125. doi:10.1016/j.jcv.2011.03.006 [5] Vinjé J.Advances in laboratory methods for detection and typing of norovirus[J]. J Clin Microbiol,2015, 53: 373-381. doi:10.1128/JCM.01535-14 [6] Zheng DP, Widdowson MR. Molecular epidemiology of genogroup II-genotype 4 noroviruses in the United States between 1994 and 2006[J]. J Clinical Microbiol, 2010, 48(1): 168-177. [7] Bull RA, Hansman GS, Clancy LE, et al. Norovirus recombination in ORF1/ORF2 overlap[J]. Emerg Infect Dis, 2005, 11(7): 1079-1085. [8] Eden JS, Tanaka MM, Boni MF, et al. Recombination within the pandemic norovirus GII.4 lineage[J]. J Virol, 2013, 87(11): 6270-6282. doi:10.1128/JVI.03464-12 [9] Nahar S,Afrad MH,Matthijnssens J, et al. Novel intergenotype human norovirus recombinant GII.16/GII.3 in Bangladesh[J]. Infect Genet Evol,2013, 20: 325-329. doi:10.1016/j.meegid.2013.09.021 [10] Fumian TM,da Silva Ribeiro de Andrade J,Leite JP, et al. Norovirus recombinant strains isolated from gastroenteritis outbreaks in Southern Brazil, 2004-2011[J]. PLoS One,2016, 11(4): e0145391. doi:10.1371/journal.pone.0145391 [11] Arana A,Cilla G,Montes M,et al. Genotypes, recombinant forms, and variants of norovirus GII.4 in Gipuzkoa (Basque Country, Spain), 2009-2012[J]. PLoS One,2014, 9(6): e98875. doi:10.1371/journal.pone.0098875 [12] Medici MC,Tummolo F,Martella V, et al. Novel recombinant GII.P16_GII.13 and GII.P16_GII.3 norovirus strains in Italy[J]. Virus Res,2014, 88: 14214-14215. doi:10.1016/j.virusres.2014.04.005 [13] Matsushima Y,Shimizu T,Ishikawa M, et al. Complete genome sequence of a recombinant GII.P16-GII.4 norovirus detected in Kawasaki City,Japan, in 2016[J]. Genome Announc,2016, 4(5). pii: e01099-16. doi:10.1128/genomeA.01099-16 [14] Iritani N,Kaida A,Abe N,et al. Increase of GII.2 norovirus infections during the 2009-2010 season in Osaka City, Japan[J]. J Med Virol,2012, 84(3): 517-525. doi:10.1002/jmv.23211 [15] Yao D, Chen JF, Ye W, et al. Etiology and genotype features analysis of an acute gastroenteritis outbreak associated with Norovirus GII.17[J]. Chin J Zoonoses, 2016, 32(7): 641-643.doi:10.3969/j.issn.1002-2694.2016.07.010 (in Chinese"> doi:10.3969/j.issn.1002-2694.2016.07.010 (in Chinese) 姚栋, 陈静芳, 叶文, 等. 一起诺如病毒GⅡ.17型引起的急性胃肠炎病原学诊断及基因特征分析[J]. 中国人兽共患病学报, 2016, 32(7): 641-643. doi:10.3969/j.issn.1002-2694.2016.07.010
2017, Vol. 33 
