Campylobacter jejuni AhpC protein B cell antigen epitope prediction and antigenic analysis
CHEN Hao-hao1, 2, LI Xu-sheng1, GUO Fang-ming1, DING Ming-xing1, HU Ye2, LOU Hong-qiang1, 2
1. Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, Jinhua 321007, China; 2. Institute of Zoonosis, Jinhua Polytechic, Jinhua 321007, China
Abstract：To provide evidence for Campylobacter jejuni(C.jejuni) vaccine research, the B cell epitope of AhpC protein were predicted, and the antigenicity was analyzed. AhpC was found locating in outer membrane of C.jejuni without transmembrane structure and no signal peptide by bioinformatics software TMHMM Server V2.0, SignalP 4.1. There were seven B-cell linear epitopes in AhpC predicted by IEDB. Then, the AhpC protein and chemically synthesized antigenic epitopes of C.jejuni were used as antigens, and the AhpC antibody of C.jejuni was used as the primary antibody, the antigens of predominant linear B cell epitopes were detected by ELISA and dot blot. Results showed that one epitope of B cell epitopes (AhpC4-16) was able to recognize the antibodies of C.jejuni AhpC and had strong antigenicity, indicating that could be used in the follow-up vaccine research.
[1] DuPont HL. Clinical practice. Bacterial Diarrhea[J].N Engl J Med, 2009, 361: 1560-1569. doi:10.1056/NEJMcp0904162 [2] Jorgensen F, Bailey R, Williams S, et al. Prevalence and numbers of Salmonella and Campylobacter spp. on raw, whole chickens in relation to sampling methods[J]. Int J Food Microbiol, 2002, 76(1/2): 151-164. doi:10.1016/S0168-1605(02)00027-2 [3] Aboutaleb N, Kuijper EJ, van Dissel JT. Emerging infectious colitis[J]. Curr Opin Gastroenterol, 2014, 30(1): 106-115. doi:10.1097/MOG.0000000000000030 [4] Fiedoruk K, Daniluk T, Rozkiewicz D. Conventional and molecular methods in the diagnosis of community-acquired diarrhoea in children under 5 years of age from the north-eastern region of Poland[J]. Int J Infect Dis, 2015, 37: 145-151. doi:10.1016/j.ijid.2015.06.028 [5] Ivanova K, Marina M, Petrov P, et al. Campylobacteriosis and other bacterial gastrointestinal diseases in Sofia, Bulgaria for the period 1987-2008[J]. Euro Surveill, 2010, 15(4): 19474. [6] Oh E, Jeon B. Role of alkyl hydroperoxide reductase (AhpC) in the biofilm formation of Campylobacter jejuni [J]. PLoS One, 2014, 9(1): e87312. doi:10.1371/journal.pone.0087312 [7] Perkins A, Poole LB, Karplus PA. Tuning of peroxiredoxin catalysis for various physiological roles[J]. Biochemistry, 2014, 53(49):7693-7705. doi:10.1021/bi5013222 [8] Maue AC, Poly F, Guerry P. A capsule conjugate vaccine approach to prevent diarrheal disease caused by Campylobacter jejuni [J].Hum Vaccin Immunother, 2014, 10(6):499-504. doi:10.4161/hv.27985 [9] Hu XJ, Han XF, Zhu DM, et al. Molecular characteristics of multidrug resistant Campylobacter jejuni and Campylobacter coli isolated from broilers[J].Chin J Zoonoses, 2015, 31(8): 694-699. doi:10.3969/j.issn.1002-2694.2015.08.002 (in Chinese) 胡欣洁,韩新锋,朱冬梅,等.肉鸡源多重耐药空肠、结肠弯曲菌的耐药分子特征[J].中国人兽共患病学报,2015,31(8): 694-699. [10] Shin E, Hong H, Oh Y, et al. First report and molecular characterization of a Campylobacter jejuni isolate with extensive drug resistance from a travel-associated human case[J]. Antimicrobial Agents Chemother, 2015, 59(10): 6670-6672. doi:10.1128/AAC.01395-15 [11] O’Riordan AA, Morales VA, Mulligan L, et al. Alkyl hydroperoxide reductase: a candidate Helicobacter pylorivaccine [J].Vaccine, 2012, 30(26): 3876-3884. doi:10.1016/j.vaccine.2012.04.002 [12] Cao FF, Meng FL, Qiao B, et al. Cloning, expression, purification and antigenicity analysis of AhpC in Campylobacter jejuni [J].Chin J Zoonoses, 2011, 27(10): 909-913. (in Chinese) 曹芳芳,孟凡亮,乔博,等.空肠弯曲菌AhpC蛋白的克隆表达及抗原性分析[J].中国人兽共患病学报,2011,27(10): 909-913. [13] Lou HQ, Hu Y, Wang L, et al. Analysis and identification of B cell epitopes of the OMP18 from Campylobacter jejuni [J]. Chin J Zoonoses, 2014, 30(7): 739-742. doi:10.3969/cjz.j.issn.1002-2694.2014.07.015 (in Chinese) 楼宏强,胡野,王岚,等.空肠弯曲菌主要外膜蛋白 OMP18的B 细胞抗原表位分析及功能鉴定[J].中国人兽共患病学报,2014,30(7): 739-742. [14] Lou HQ, Gao SH, Li XS, et al. Establishment of ELISA method for detecting Campylobacter jejuni based on the B-cell antigen epitope of OMP18[J]. Chin J Zoonoses, 2016, 32(10): 889-892. doi:10.3969/j.issn.1002-2694.2016.010.008(in Chinese) 楼宏强,高素华,李旭升,等.基于OMP18的B细胞抗原表位的空肠弯曲菌ELISA检测方法的建立[J].中国人兽共患病学报,2016,32(10): 889-892.
2017, Vol. 33 
