Characteristics of drug resistance and molecular typing for Salmonella in diarrhea patients from four hospitals in Shenzhen
LONG Dong-ling1, WEI Jie-hong2, WU Jing-song3, HAO Hao-yu4, JIANG Shu-ping5, LI Jian-hua1, ZHUO Fei1
1. Luohu District Center for Disease Control and Prevention, Shenzhen 518020, China; 2. Department of Laboratory Medicine, the Fifth People’s Hospital of Shenzhen, Shenzhen 518020, China; 3. Department of Laboratory Medicine, Shenzhen People’s Hospital, Shenzhen 518020, China; 4. Department of Laboratory Medicine, Shenzhen Yuandong Maternity and Child Healthcare Hospital, Shenzhen 518020, China; 5. Department of Laboratory Medicine, Shenzhen Luohu Maternity and Child Healthcare Hospital, Shenzhen 518020, China
Abstract：We analyzed the antimicrobial resistance and pulsed field gel electrophoresis(PFGE) patterns of Salmonella strains of infectious diarrhea isolated four hospitals in Shenzhen during 2012-2016. A total of 137 Salmonella strains collected in 4 hospitals in Shenzhen were identified by serotyping, micro-dilution method was used to detect the antimicrobial susceptibility of the isolates. Pulsed field gel-electrophoresis(PFGE) was used for molecular typing and cluster analysis of S. typhimurium and S. enterica strains. Results showed that 137 strains of Salmonella were divided into 30 kinds of serotype. S. typhimurium was the predominant serotype. The drug susceptibility test indicated that the antibiotic resistance of the strains to chloromycetin, ampicillin and amoxicillin were more serious than other drugs, the resistance to penicillins, cephalosporins, aminolycosides and macrolides showed increase trends year by year. Of 49 strains of S. typhimurium were divided 38 molecular patterns by PFGE, with similarity ranged from 65.5%-100%, TY18 was the main PFGE pattern. The 27 strains of S. enterica were divided into 7 molecular patterns by PFGE, with similarity ranged from 61.8%-100%, EN7 was the main PFGE pattern. The status of drug resistance of infectious diarrhea isolates was rather severe, PFGE patterns showed diversity, suggesting the diarrhea cases were distributed sporadically. Partial PFGE patterns and its corresponding antidrug spectrum have certain aggregation relationship.
龙冬玲, 韦洁宏, 吴劲松, 郝红雨, 蒋淑萍, 黎剑华, 卓菲. 深圳地区四家医院感染性腹泻来源沙门菌耐药性及分子分型研究[J]. 中国人兽共患病学报, 2017, 33(10): 897-902.
LONG Dong-ling, WEI Jie-hong, WU Jing-song, HAO Hao-yu, JIANG Shu-ping, LI Jian-hua, ZHUO Fei. Characteristics of drug resistance and molecular typing for Salmonella in diarrhea patients from four hospitals in Shenzhen. Chinese Journal of Zoonoses, 2017, 33(10): 897-902.
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2017, Vol. 33 
