Aminoglycoside resistance genes of Acinetobacter baumannii
GUO Pu1, QIAO Yan2, LI Jing1
1.Department of Clinical Medical Laboratory, the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004, China; 2.Department of Infeetions Diseases,the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004,China
Abstract:To investigate the distribution of the resistance genes of Acinetobacter baumannii to aminoglycoside, 48 strains of extensively drug resistant Acinetobacter baumannii were collected from the First Affiliated Hospital of Bengbu Medical College from January to December,2015. The drug sensitivity test and identification were performed by VITEK 2 compact automatic microorganism instrument. Twelve aminoglycosides modifying enzymes, three 16SrRNA methylase genes and efflux pump abeB gene were detected from these isolates by PCR. Results showed that among these experimental 16 genes, aac(6')-Ib gene was detected from 19 of 48 isolates (39.6%), both armA and adeB genes were 43 (89.6%), ant(3″)-Ia gene was from 5 (10.4%), while the other genes were not found.And more than two gene types were amplified from 39 of 48 strains (81.3%). In conclusion,the aac(6')-Ib, armA gene and efflux pump adeB may play a key role in drug resistance to aminoglycosides antibiotics of Acinetobacter Baumanni in our hospital.
[1] Hang J, Chen EZ, Qiu HP, et al. Sources of multidrug-resistant Acinetobacter baumannii and its role in respiratory tract colonization and nosocomial pneumonia in intensive care unit patients[J]. Chin Med(Engl), 2013, 126(10): 1826-1831. [2] Hu FP, Zhu DM, Wang F, et al. 2014 CHINET surveillance of bacterial resistance in China[J]. Chin J Infect Chemother, 2015, 15(5): 401-410.doi:10.3969/j.issn.1009-7708.2015.05.001(in Chinese) 胡付品,朱德妹,汪复,等.2014年中国CHINET细菌耐药性监测[J].中国感染与化疗杂志,2015,15(5):401-410. [3] Inchai J, Liwsrisakun C, Theerakittikul T, et al. Risk factors of multidrug-resistant, extensively drug-resistant and pandrug-resistant Acinetobacter baumannii ventilator-associated pneumonia in a Medical Intensive Care Unit of University Hospital in Thailand[J]. J Infect Chemother, 2015, 21(8): 570-574.doi:10.1016/j.jiac.2015.04.010 [4] Nie L, Lv Y, Yuan M, et al. Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing, China[J]. Acta Pharm Sin B,2014, 4(4): 295-300. doi:10.1016/j.apsb. 2014.06.004. [5] Kuai SG, Huang LH, Pei H, et al. Study on the molecular mechanism of aminoglycoside resistance to Acinetobacter baumannii [J]. Lab Med, 2012, 27(8): 619-623.doi:10.3969/j.issn.1673-8640.2012.08.001 (in Chinese) 蒯守刚,黄利华,裴豪,等.鲍曼不动杆菌对氨基糖苷类药物耐药机制研究[J].检验医学,2012,27(8):619-623. [6] Fu Q, Ye H, Liu S. Risk factors for extensive drug-resistance and mortality in geriatric inpatients with bacteremia caused by Acinetobacter baumannii [J]. Am J Infect Control, 2015, 43(8): 857-860.doi:10.1016/j.ajic.2015.03.033. [7] Chen BY,He LX,Hu BJ. Consensus of the Chinese specialists for diagnosis, treatment & control of Acinetobacter baumannii infection[J].Natl Med J China,2012, 92(2): 76-85. doi:10.3760/cma.j.issn.0376-2491.2012.02.002 (in Chinese) 陈佰仪,何礼贤,胡必杰,等.中国鲍曼不动杆菌感染诊治与防控专家共识[J]. 中华医学杂志, 2012, 92(2): 76-85. [8] Xiao SZ,Han LZ,Chu HQ, et al. Detection of aminoglycoside resistance related genes in multidrug-resistant Acinetobacter baumannii isolated from a single institute of Shanghai, China[J]. Panminerva Med,2015, 57(1): 49-53. [9] Yu H, Liu H, Huang WF, et al. Sichuan Provincial Antimicrobial Resistant Investigation Net 2012 annual report: Bacterial drug resistance surveillance in Sichuan[J]. Chin J Antibiotics, 2014, 39(5): 332-337. doi:10.3969/j.issn.1001-8689.2014.05.003 (in Chinese) 喻华,刘华,黄文芳,等.四川省细菌耐药监测网2012年细菌耐药性监测[J].中国抗生素杂志,2014,39(5):332-337. [10] Guo P, Qiao Y, Zhang HT, et al. Surveillance of antibiotic resistance in clinical isolates from the First Affiliated Hospital of Bengbu Medical College during 2014[J]. Chin J Antibiotics, 2015,40(10): 760-765. doi:10.3969/j.issn.1001-8689.2015.10.009 (in Chinese) 郭普,乔艳,张海涛,等.2014年蚌埠医学院第一附属医院病原菌耐药性监测[J].中国抗生素杂志,2015,40(10):760-765. [11] Aghazadeh M,Rezaee MA,Nahaei MR, et al. Dissemination of aminoglycoside-modifying enzymes and 16S rRNA methylases among Acinetobacter baumannii and Pseudomonas aeruginosa isolates[J]. Microb Drug Resist,2013, 19(4): 282-288.doi:10.1089/mdr.2012.0223 [12] Tang CG, Li QH, Lin T. Spread of aminoglycoside modifying enzyme gene: aac(2')-Ib in drug-resistant A. baumannii [J]. Chin J Antibiotics, 2014, 39(11): 844-848. doi:10.3969/j.issn.1001-8689.2014.11.009 (in Chinese) 唐朝贵,李前辉,林涛,等.氨基糖苷类修饰酶基因aac(2')-Ib型在耐药鲍曼不动杆菌中流行[J].中国抗生素杂志,2014,39(11):844-848. [13] Liu XQ, Chen J, Li H, et al. Study of aminoglycoside drug-resistant gene on the plasmid of Acinetobacter baumannii [J]. Chin J Antibiotics, 2012, 37(5): 335-356. doi:10.3969/j.issn.1001-8689.2012.05.003 (in Chinese) 刘晓庆,陈娇,李华,等.鲍曼不动杆菌质粒上氨基糖苷类耐药基因的研究[J].中国抗生素杂志,2012,37(5):335-356.