Infection of Babesia in domestic animals in Western Yunnan Province, China
HUANG Tao-hua1,2, GAO Zi-hou1, JIANG Bao-gui3, ZHAO Wen-hong1, SU Chao1, JIA Na3, ZHANG Qing1, JIANG Jia-fu3, DU Chun-hong1
1. Yunnan Institute of Endemic Diseases Control and Prevention, Dali 671000, China;
2. School of Public Health, Dali University, Dali 671000, China;
3. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 1000071, China
Abstract:The objective of this study was to investigate Babesia infection among domestic animals in Western Yunnan Province and provide scientific evidence for developing control measures. A total of 1 073 domestic animals blood samples (274 cattle, 395 sheep, 354 dogs, 33 horses and 17 donkeys) were collected in 12 counties in Western Yunnan Province. Genomic DNA was extracted and a near full-length 18S rRNA gene sequence of Babesia was amplified by using nested PCR. Babesia species was identified by Blast program and phylogenetic tree. It was indicated that 50 samples were infected with Babesia, belonging to 5 species and with the infection rate of 4.66%. Among 274 cattle blood samples, 11 were infected with Babesia (4.01%). Four of them were Babesia bovis and seven of them were Babesia bigemina. Among 395 sheep blood samples, 38 were infected with Babesia (9.62%), 37 of them were Babesia odocoilei-like parasites and 1 of them was Babesia capreoli-like parasites. Horses and donkeys were negative. In conclusion, domestic animals in Western Yunnan Province are infected with many kinds of Babesia, which threaten stock raising development and human health. It is necessary to strengthen prevention of babesiosis and investigate infection rate of babesiosis in human.
[1] Wu GL. Human parasitology[M]. 4 th ed, Beijing: People’s Medical Publishing House,2013:233-237. (in Chinese)
吴观陵.人体寄生虫学[M].4版.北京:人民卫生出版社,2013:233-237.
[2] Vannier E, Gewurz BE, Krause PJ. Human babesiosis[J].Infect Dis Clin North Am, 2008, 22(3):469-488. doi:10. 1016/j. idc.2008.03.010
[3] Man SQ, Qiao K, Cui J, et al. A case of human infection with a novel Babesia species in China[J]. Infect Dis Poverty, 2016, 5(28): 1-6. doi:10.1186/s40249-016-0121-1
[4] Chen Z, Yang XJ, Yang XH, et al. Geographical distribution and fauna of Chinese ticks[J].Sichuan J Zool, 2008, 27(5): 820-823. (in Chinese)
陈泽,杨晓军,杨晓红,等.中国蜱类地理分布及区系分析[J].四川动物,2008,27(5):820-823.
[5] Wang HX. A case of human babesiosis in Southwest region of China[J]. Int Med Parasit Dis, 2012, 39(3): 190-192. (in Chinese)
王惠萱.昆明地区巴贝西虫病一例分析[J].国际医学寄生虫病杂志,2012, 39(3):190-192.
[6] Li JF, Meng DB, Wang QF, et al. Discovery of human babesiosis[J]. Chin J Vet Med, 1984, 10(6): 19-20. (in Chinese)
李金福,孟大邦,王庆芳,等.人巴贝斯焦虫病的发现[J].中国兽医杂志,1984.10(6):19-20.
[7] Zhou X, Li SG, Chen SB, et al. Co-infections with Babesia microti and Plasmodium parasites along the China-Myanmar border[J]. Infec Dis Poverty, 2013, 2(1): 1-7. doi:10.1186/2049-9957-2-24
[8] Niu QL, Liu ZJ, Yu PF, et al. Genetic characterization and molecular survey of Babesia ovata in cattle, dairy cattle and yaks in China[J]. Parasit Vectors, 2015, 8(1): 1-13. doi:10.1186/S13071-015-1110-0
[9] Lü WX, Lü WS, Zhang QC, et al. Detection of the tick-borne diseases and research of their distribution characteristics in China mainland[J]. Chin J Vet Sci Technol, 1995, 25(8): 13-16. (in Chinese)
吕文祥,吕文顺,张其才,等.我国牛蜱传性血液原虫的虫种调查和分布特征的研究[J].中国兽医科技,1995,25(8): 13-16.
[10] Kawabuchi T, Tsuji M, Sado A, et al. Babesia microti -like parasites detected in feral raccoons ( Procyon lotor ) captured in Hokkaido, Japan[J]. J Vet Med Sci, 2005, 67(8): 825-827.
[11] Huang TH, Gao ZH, Du CH. Research progress on epidemiology of host animals and vectors of babesiosis[J]. Chin Trop Med, 2015, 159(9): 1151-1154. (in Chinese)
黄涛华,高子厚,杜春红.巴贝虫病宿主动物和媒介流行病学研究进展[J].中国热带医学,2015,159(9):1151-1154.
[12] Han RL, Gong QG. Prevention and treatment of cow babesiosis in Dali[J]. Chin J Anim Husb Vet Med, 2005, 5: 46-47. (in Chinese)
韩荣林,龚启耕.大理市暴发奶牛焦虫病的诊治[J].畜牧兽医科技信息,2005,5:46-47.
[13] He AF. Diagnosis, prevention and treatment of bovine babesiosis[J]. Yunnan J Ani Sci Vet Med, 2006, 6: 17-18. (in Chinese)
和爱芬.牛巴贝斯焦虫病的诊断与防治措施[J].云南畜牧兽医,2006,6:17-18.
[14] He ZX. Diagnosis and treatment of bovine babesiosis in livestock farm[J]. China Anim Husband Vet Abstracts, 2014, 30(10): 161-161. (in Chinese)
和泽贤.养牛场巴贝斯焦虫病的诊疗体会[J].中国畜牧兽医文摘,2014,30(10):161.
[15] Shi FH.Zhu CF,Yang JZ,et al. Diagnosis, prevention and treatment of bovine babesiosis in a livestock farm[J]. Yunnan J Anim Sci Vet Med, 2016, 3: 20-21. (in Chinese)
石凤海,祝常芬,杨静竹,等.某牛场牛巴贝斯焦虫病的诊断与防治[J].云南畜牧兽医,2016, 3: 20-21.
[16] Schoelkopf L, Hutchinson CE, Bendele KG, et al. New ruminant hosts and wider geographic range identified for Babesia odocoilei (Emerson and Wright 1970)[J]. J Wildl Dis, 2005, 41(4): 683-690. doi:10.7589/0090-3558-41.4.683
[17] Bartlett SL, Madi NA, Messick JB, et al. Diagnosis and treatment of Babesia odocoilei in captive reindeer ( Rangifer tarandus tarandus ) and recognition of three novel host species[J]. J Zoo Wildl Med, 2009, 40(1): 152-159. doi:10.1638/2008-0011.1
[18] Xu Q, Sun YL, Wang SQ, et al. Treatment and experience of a case of canine babesiosis[J]. Shandong J Anim Sci Vet Med, 2014, 35(8): 39-40. (in Chinese)
徐谦,孙玉林,王生奎,等.一例犬巴贝斯虫病的诊治与体会[J].山东畜牧兽医,2014, 35(8):39-40.
[19] Wang RQ, Liu YZ. Diagnosis and analysis of canine babesiosis[J]. Heilongjiang Anim Sci Vet Med, 2011, 5: 115-116. (in Chinese)
王荣琼,刘永张,等.犬感染巴贝斯虫病例诊断与分析[J].黑龙江畜牧兽医,2011,5:115-116.
[20] Yang CF, Wang JJ, He SW, et al. Diagnosis and treatment of goat babesiosis[J]. Livestock Poul Industry, 2009, 6: 70-71. (in Chinese)
杨成飞,王金菊,何守维,等.山羊巴贝斯焦虫病的诊治[J].畜禽业,2009,6:70-71.
[21] Yin KN, Yang SH, Lei KG, et al. Diagnosis and treatment of pig babesiosis[J]. Chin Livestock Poultry Breed, 2014, 2: 103. (in Chinese)
尹可能,杨善海,雷开贵,等.猪焦虫病诊断与治疗体会[J].中国畜禽种业,2014,2:103.