Efficacy of Albizia julibrissin total saponins against Trichinella spiralis in mice
ZHU Xue-xue1, CAI Wei-wei1, GONG Lei-lei1, LEI Yue-yue1, TAN Fang-gen1, ZHOU Yong-hua2, QIU Li-ying1
1. Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China; 2. Jiangsu Institute of Parasitic Diseases, Wuxi 214122, China
Abstract：We investigated the therapeutic effect of Albizia julibrissin total saponins on mice infected with Trichinella spiralis. Thirty-six ICR mice infected with Trichinella spiralis were randomly divided into 6 groups (each mouse infected with 300 T. spiralis), 6 mice in each. Group Ⅰ: infected non-treated group (intestinal phase); group Ⅱ: received Albizia julibrissin total saponins group (intestinal phase); group Ⅲ: received albendazole group (intestinal phase); group Ⅳ: infected non-treated group (muscular phase); group Ⅴ: received Albizia julibrissin total saponins group (muscular phase); group Ⅵ: received albendazole group (muscular phase). Mice of Ⅰ, Ⅱ, Ⅲ group were administered on the second days post-infection(dpi) and continued for 3 days. Mice in these groups were sacrificed 7th dpi and adult worms recovered from the small intestine were counted. Mice of Ⅳ, Ⅴ, Ⅵ group were administered on the 7th dpi and continued for 14 d. The mice were sacrificed on 40th dpi, and the muscle larvae were counted. HE staining counts muscle larvae and the expression of IL-1β, IL-6, TNF-α and COX-2 in the diaphragm were detected by immunohistochemistry. Results showed that the number of adult worms and larva in groups received Albizia julibrissin total saponins and albendazole were significantly lower than that of infected non-treated group (P<0.01). The worms reduction rate was 70.34% and 80.02% respectively, and the larva were 65.60% and 90.66% respectively. Results of HE staining showed the number of encysted larval and the expression of inflammatory cell were significantly reduced. The expression of IL-1β, IL-6, TNF-α and COX-2 was decreased in drug-treated groups. In conclusion, the total saponins of Albizia julibrissin showed adequate efficacy on Trichinella spiralis adults and encapsulated larva. Although the effect is slightly inferior to albendazole, as traditional Chinese medicine extract, it is less toxic.
朱雪雪, 蔡维维, 龚蕾蕾, 雷月月, 谭方根, 周永华, 邱丽颖. 合欢皮总皂苷治疗旋毛虫感染小鼠的疗效观察[J]. 中国人兽共患病学报, 2018, 34(1): 39-43.
ZHU Xue-xue, CAI Wei-wei, GONG Lei-lei, LEI Yue-yue, TAN Fang-gen, ZHOU Yong-hua, QIU Li-ying. Efficacy of Albizia julibrissin total saponins against Trichinella spiralis in mice. Chinese Journal of Zoonoses, 2018, 34(1): 39-43.
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2018, Vol. 34 
