IL-33 deficiency promotes M1 polarization of mouse peritoneal macrophages infected with Toxoplasma gondii
HE Xiao-li1, WU Lin-qing2, XU Wei-qun1, YAN Cai-ling3, LIN Jun-jin3, ZHANG Lu-rong4, ZHANG Tao2
1. Experimental Teaching Center of Basic Medical Sciences, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China; 2. Department of Immunology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China; 3. Scientific Research Center of Basic Medical Sciences, School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China; 4. Fujian Platform for Medical Research at the First Affiliated Hospital, Fujian Key Laboratory of Individualized Active Immunotherapy and Key Laboratory of Radiation Biology, Fujian province, Fuzhou350005, China
Abstract：We investigated the effect of IL-33 knockout on the polarization of peritoneal macrophages from mouse with acute Toxoplasma infection, in order to uncover the function of IL-33 knockout in mice macrophages with acute Toxoplasma infection. pMφ was isolated from C57BL/6 wild type and IL-33 deficient mice and divided into Toxoplasma infected group and control group respectively. Infection rate of pMφ was determined; the mRNA of iNOS, Arg-1, IL-1, IL-10 and IL-12 were analyzed by real-time PCR; the secretion of IL-12, TNF-α, IL-10 and NO were detected by ELISA and Griess method respectively; the surface molecules (CD80, CD86, CD206, TLR4, TLR2, MHCⅡ) were analyzed by flow cytometry. Results showed that the infection rate of pMφ was deceased in IL-33-/- mice compared with wild-type mice (t=-2.49,P<0.05); the expression of M1 makers NO(t=29.71,P<0.05), MHCⅡ(t=19.05,P<0.05), CD86 and TLR4(t=8.34,P<0.05) were increased (P<0.01) while the M2 maker (CD206) was down-regulated in IL-33-/- mice infected with Toxoplasma than that in the wild-type infected group; the secretion of NO, IL-10, TNF-α and the expression of MHCⅡand CD86 were higher in infected group of both IL-33-/- and wild-type mice compared with uninfected control group respectively (P<0.01). Results suggest that IL-33 knockout promote the secretion of NO and the expression of MHCⅡ(F=14.88,P<0.05), CD86 and TLR4 via driving polarization of M1 macrophages, thereby enhancing the immune protection in acute Toxoplasma infection.
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