结核分枝杆菌RD区蛋白研究进展

doi:10.3969/j.issn.1002-2694.2018.00.076

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摘要由病原菌结核分枝杆菌(Mycobacterium tuberculosis,Mtb)引发的结核病是一种严重危害人类健康的传染病。根据世界卫生组织的《全球结核病报告2017》年报指出:2016年新增结核病感染者1 040万例,其中死亡人数达到167万例,死于HIV共感染的人数37万。随着多耐药性菌株和HIV共感染等出现,使得结核病的预防和治疗形式愈加严峻,积极研发新型结核疫苗十分必要。结核分枝杆菌RD区编码蛋白在结核病新型疫苗研发及新型诊断方法建立中具有重要的潜在意义及应用价值。本文主要介绍结核分枝杆菌基因组RD区及其编码蛋白的研究进展。

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	李文彬
	万康林

关键词 ：结核分枝杆菌, RD区, 蛋白, 诊断

Abstract：Tuberculosis(TB),caused by pathogenic Mycobacterium tuberculosis(M. tuberculosis),is a infectious disease that seriously harms human health. According to Global Tuberculosis Report 2017(World Health Organization Tuberculosis Dataand Statistics, 2017),there are 10.4 million new cases and 1.67 million people died from TB,with 370 000 deaths while HIV-positive.The emergence of multi-drug-resistant(MDR)strains and coinfection with HIV lead to the situation of TB treatment is still more serious, it is necessary to develop novel types of vaccine against tuberculosis.The Mycobacterium tuberculosis RD region coding protein in the development of new TB vaccines and the establishment of new diagnostic methods have important potential significance and application value. This review focuses on the genomic characteristics of RD region and the function of produtcts.

Key words： Mycobacterium tuberculosis RD region protein diagnosis

收稿日期: 2017-08-15

PACS:

R378.91

基金资助:国家科技重大专项(No.2018ZX10731301-002)资助

通讯作者: 万康林,Email: wankanglin@icdc.cn

引用本文:

李文彬, 万康林. 结核分枝杆菌RD区蛋白研究进展[J]. 中国人兽共患病学报, 2018, 34(4): 362-371. LI Wen-bin, WAN kang-lin. Research progress on regions of differences protein in tuberculosis. Chinese Journal of Zoonoses, 2018, 34(4): 362-371.

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http://www.rsghb.cn/CN/10.3969/j.issn.1002-2694.2018.00.076 或 http://www.rsghb.cn/CN/Y2018/V34/I4/362

[1] Barberis I, Bragazzi NL, Galluzzo L,et al.The history of tuberculosis: from the first historical records to the isolation of Koch's bacillus[J]. J Prev Med Hyg, 2017, 58(1): E9-E12.
[2] Pounder JI, Anderson CM, Voelkerding KV,et al.Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis[J]. Diagn Microbiol Infect Dis, 2010, 67(1): 101-105. DOI: 10.1016/j.diagmicrobio.2009.12.014
[3] Cole ST, Brosch R, Parkhill J,et al.Deciphering the biology of Mycobacterium tuberculosis from thecomplete genome sequence[J]. Nature, 1998, 393(6685): 537-44. DOI:10.1038/31159
[4] 丁志鑫,车南颖,张旭霞,等. 结核分枝杆菌差异区基因功能研究及其应用进展[J]. 临床肺科杂志, 2012, 17(3): 505-508. DOI:10.3969/j.issn.1009-6663.2012.03.060
[5] Brosch R, Gordon SV, Billault A,et al.Use of a Mycobacterium tuberculosis H37Rv bacterial artificial chromosome (BAC) library for genome mapping, sequencing and comparative genomics[J]. Infect Immun, 1998, 66(5): 2221-2229.
[6] Philipp WJ, Nair S, Guglielmi G,et al. Physical mapping of Mycobacterium bovis BCG pasteur reveals differences from the genome map of Mycobacterium tuberculosis H37Rv and from M. bovis[J]. Microbiology, 1996, 142( Pt 11)(11): 3135. DOI:10.1099/13500872-142-11-3135
[7] Behr MA, Wilson MA, Gill WP,et al.Comparative genomics of BCG vaccines by whole-genome DNA microarray[J]. Science, 1999, 284(5419): 1520-3. DOI: 10.1126/science.284.5419.1520
[8] 池水晶,宝福凯,柳爱华.结核分枝杆菌比较基因组学研究进展[J].科技通报, 2013, (9): 40-45. DOI:10.13774/j.cnki.kjtb.2013.09.029
[9] Fan X, Gao Q, Fu R.DNA vaccine encoding ESAT-6 enhances the protective efficacy of BCG against Mycobacterium tuberculosis infection in mice[J]. Scand J Immunol, 2007, 66(5): 523-8. DOI:10.1111/j.1365-3083.2007.02006.x
[10] Kalra M,Grover A,Mehta N,et al.Supplementation with RD antigens enhances the protective efficacy of BCG in tuberculous mice[J]. Clin Immunol, 2007, 125(2): 173-83. DOI:10.1016/j.clim.2007.07.007
[11] Renshaw PS, Panagiotidou P, Whelan A,et al.Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence[J]. J Biol Chem, 2002, 277(24): 21598-603. DOI:10.1074/jbc.M201625200
[12] 鲍一歌,秦紫芳,鲍朗.结核杆菌基因组RD1区毒力蛋白分泌相关基因Rv3871的克隆、表达及生物信息学分析[J]. 南方医科大学学报, 2009, 29(12): 2371-2374. DOI:10.3321/j.issn:1673-4254.2009.12.004
[13] Tiwari B,Soory A,Raghunand TR.An immunomodulatory role for the Mycobacterium tuberculosis region of difference 1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873)[J]. FEBS J, 2014, 281(6): 1556-70. DOI:10.1111/febs.12723
[14] Berthet FX, Rasmussen PB, Rosenkrands I,et al.A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10)[J]. Microbiology, 1998,144(Pt 11): 3195-3203. DOI:10.1099/00221287-144-11-3195
[15] Zhang M, Chen JM, Sala C,et al.EspI regulates the ESX-1 secretion system in response to ATP levels in Mycobacterium tuberculosis[J]. Mol Microbiol, 2014, 93(5): 1057-65. DOI: 10.1111/mmi.12718
[16] Champion PA, Stanley SA, Champion MM,et al.C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis[J]. Science, 2006, 313(5793): 1632-6. DOI:10.1126/science.1131167
[17] Singh PK, Saxena R, Tiwari S,et al.RD-1 encoded EspJ protein gets phosphorylated prior to affect the growth and intracellular survival of mycobacteria[J]. Sci Rep, 2015, 5: 12717. DOI: 10.1038/srep12717
[18] 李浩,徐俊杰,陈薇. 结核分枝杆菌RD-1区编码蛋白的结构和功能[J]. 生物化学与生物物理进展, 2009, 36(10): 1260-1266. DOI: 10.3724/SP.J.1206.2009.00187
[19] Raghavan S,Manzanillo P,Chan K,et al.Secreted transcription factor controls Mycobacterium tuberculosis virulence[J]. Nature, 2008, 454(7205): 717-21. DOI: 10.1038/nature07219
[20] Kozak RA, Alexander DC, Liao R,et al.Region of difference 2 contributes to virulence of Mycobacterium tuberculosis[J]. Infect Immun, 2011, 79(1): 59-66. DOI: 10.1128/IAI.00824-10
[21] Ramage HR, Connolly IE, Cox JS.Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution[J]. PLoS Genet, 2009, 5(12): e1000767. DOI: 10.1371/journal.pgen.1000767
[22] 孙林,刘艳,闵晨雨,等. 结核分枝杆菌NrdF1、PE_PGRS35、Rv1985c和Rv1986的原核表达及检测牛结核病抗体之应用[J].中国人兽共患病学报, 2014, 30(8): 782-786. DOI: 10.3969/j.issn.1002-2694.2014.08.002
[23] Sha S, Shi X,Deng G,et al.Mycobacterium tuberculosis Rv1987 induces Th2 immune responses and enhances Mycobacterium smegmatis survival in mice[J]. Microbiol Res, 2017, 197: 74-80. DOI: 10.1016/j.micres.2017.01.004
[24] Yaseen I,Kaur P, Nandicoori VK,et al.Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3[J]. Nat Commun, 2015, 6: 8922. DOI: 10.1038/ncomms9922
[25] Fan X,Abd Alla AA,Xie J.Distribution and function of prophage phiRv1 and phiRv2 among Mycobacterium tuberculosis complex[J]. J Biomol Struct Dyn, 2016, 34(2): 233-238. DOI: 10.1080/07391102.2015.102260
[26] Kwok HF,Scott CJ,Snoddy P,et al.Expression and purification of diagnostically sensitive mycobacterial (Mycobacterium bovis) antigens and profiling of their humoral immune response in a rabbit model[J]. Res Vet Sci, 2010, 89(1): 41-7. DOI: 10.1016/j.rvsc.2009.12.015
[27] Rosenkrands I,Aagaard C,Weldingh K,et al.Identification of Rv0222 from RD4 as a novel serodiagnostic target for tuberculosis[J]. Tuberculosis, 2008, 88(4): 335-343. DOI: 10.1016/j.tube.2007.12.001
[28] 张慧涨,范齐文,杨华,等. Rv0222/ESAT-6融合蛋白对结核病的诊断价值[J]. 中国临床医学, 2014, (6): 636-639.
[29] 王庆,杨华,金瑞良,等.结核分枝杆菌重组蛋白Rv0222的表达及血清学鉴定[J].中国防痨杂志, 2012, 34(1): 36-39.
[30] Rao KR,Kauser F,Srinivas S,et al.Analysis of genomic downsizing on the basis of region-of-difference polymorphism profiling of Mycobacterium tuberculosis patient isolates reveals geographic partitioning[J]. J Clin Microbiol, 2005, 43(12): 5978-82. DOI:10.1128/JCM.43.12.5978-5982.2005
[31] Zhang MM,Zhao JW,Sun ZQ,et al.Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis[J]. Clin Dev Immunol, 2012, 2012(4): 738043. DOI: 10.1155/2012/738043
[32] Rabiee-Faradonbeh M, Darban Sarokhalil D,Feizabadi MM,et al.Cloning of the Recombinant Cytochrome P450 Cyp141 Protein of Mycobacterium tuberculosis as a Diagnostic Target and Vaccine Candidate[J]. Iran Red Crescent Med J, 2014, 16(11):e18001. DOI: 10.5812/ircmj.18001
[33] 刘民强. 结核分枝杆菌差异区RD6基因Rvl515c促进胞内存活及其分子机理研究[D]. 西南大学, 2015.
[34] Alkhodari NY,Alattiyah R,Mustafa AS.Identification, diagnostic potential, and natural expression of immunodominant seroreactive peptides encoded by five Mycobacterium tuberculosis-specific genomic regions[J]. Clin Vaccine Immunol, 2011, 18(3): 477-482. DOI: 10.1128/CVI.00405-10
[35] 曹廷明,贾红彦,古淑香,等. 结核分枝杆菌Rv2352c基因的克隆表达及其抗原活性鉴定[J].中国防痨杂志,2010,32(10):25-30.
[36] 张红梅. 结核分支杆菌特异的PE/PPE蛋白应用于结核病血清学诊断的初步研究[D]. 复旦大学, 2006. DOI: 10.7666/d.y1022386
[37] Mohanty S,Molin MD,Ganguli G,et al.Mycobacterium tuberculosis EsxO (Rv2346c) promotes bacillary survival by inducing oxidative stress mediated genomic instability in macrophages[J]. Tuberculosis, 2016, 96: 44-57. DOI: 10.1016/j.tube.2015.11.006
[38] He XY,Zhuang YH,Zhang XG,et al.Comparative proteome analysis of culture supernatant proteins of Mycobacterium tuberculosis H37Rv and H37Ra[J]. Microbes Infect, 2003, 5(10): 851-856. DOI:10.1016/S1286-4579(03)00179-5
[39] 蒋天舒,娄加陶,周晔,等.结核分枝杆菌Rv0309抗原的重组表达、纯化和鉴定[J]. 临床军医杂志, 2007, 35(2): 165-167. DOI:10.3969/j.issn.1671-3826.2007.02.002
[40] 何秀云,李净,郝娟,等. 结核分枝杆菌Rv3618蛋白在大肠埃希菌中的表达、纯化及其血清学诊断[J].现代检验医学杂志,2010, 25(6): 27-29. DOI:10.3969/j.issn.1671-7414.2010.06.009
[41] Soo PC,Horng YT,Hsueh PR,et al.Direct and simultaneous identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by rapid multiplex nested PCR-ICT assay[J]. J Microbiol Methods, 2006, 66(3): 440-448. DOI:10.1016/j.mimet.2006.01.010
[42] Yang E,Lu Y,Xu Y,et al. Recombinant BCG coexpressing Ag85B, ESAT-6 and Rv3620c elicits specific Th1 immune responses in C57BL/6 mice[J]. Microb Pathog, 2014, 69-70(1): 53-59. DOI: 10.1016/j.micpath.2014.03.011
[43] 曹廷明,古淑香,马玙,等. 结核分枝杆菌Rv3623基因的抗原性及其多克隆抗体的制备[C]. 中华医学会. 中华医学会结核病学分会2012学术大会. 北京:中华医学会,2012:225.
[44] Ouellet H, Johnston JB, Ortiz De Montellano PR. The Mycobacterium tuberculosis cytochrome P450 system[J]. Arch Biochem Biophys, 2010, 493(1): 82-95. DOI: 10.1016/j.abb.2009.07.011
[45] 吴兴福,孙战强,徐明.结核分枝杆菌CFP10-ESAT6-Rv3425基因片段融合蛋白的表达与纯化[J]. 临床肺科杂志, 2011, 16(10): 1533-1535. DOI: 10.3969/j.issn.1009-6663.2011.10.025
[46] 孙卫国,李邦印,刘艳华,等. 结核分枝杆菌Rv3425蛋白的原核表达纯化及其血清学诊断价值[J]. 生物技术通讯, 2013, (5): 659-661. DOI: 10.3969/j.issn.1009-0002.2013.05.014
[47] 叶娟. 结核分枝杆菌RD12区和RD5区特异性抗原的生物信息学分析及免疫原性初步评估[D]. 河南中医学院, 2014.
[48] Tan K,Zhang J,Teng X,et al.High level of IFN-γ released from whole blood of human tuberculosis infections following stimulation with Rv2073c of Mycobacterium tuberculosis[J]. J Microbiol Methods, 2015, 114: 57-61. DOI: 10.1016/j.mimet.2015.05.007
[49] Luo W,Qu ZL,Xie Y,et al.Identification of a novel immunodominant antigen Rv2645 from RD13 with potential as a cell-mediated immunity-based TB diagnostic agent[J]. J Infect, 2015, 71(5): 534-43. DOI: 10.1016/j.jinf.2015.07.011
[50] Arlehamn CS,Sidney J,Henderson R,et al.Dissecting mechanisms of immunodominance to the common TB antigens ESAT-6, CFP10, Rv2031c (hspX), Rv2654c (TB7.7) and Rv1038c (EsxJ)[J]. J Immunol, 2012, 188(10): 5020-5031. DOI: 10.4049/jimmunol.1103556
[51] 杨丹,白雪娟,阳幼荣,等. 结核潜伏感染蛋白Rv2657cT细胞和B细胞表位的预测与分析[J]. 实用医学杂志, 2017, 33(1): 55-58. DOI: 10.3969/j.issn.1006-5725.2017.01.015
[52] Bai XJ,Liang Y,Yang YR,et al.Immune responses to latent tuberculosis antigen Rv2659c in Chinese populations[J]. J Microbiol Immunol Infect, 2015, 48(4): 381-389. DOI: 10.1016/j.jmii.2014.02.006
[53] Lu M, Xia ZY, Bao L.Enhancement of antimycobacterial Th1-cell responses by a Mycobacterium bovis BCG prime-protein boost vaccination strategy[J]. Cell Immunol, 2013, 285(1/2): 111-117. DOI: 10.1016/j.cellimm.2013.10.001
[54] Wolucka BA, Communi D.Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase[J]. FEBS J, 2006, 273(19):4435-4445. DOI: 10.1111/j.1742-4658.2006.05443.x
[55] Ahmad S,El-Shazly S,Mustafa AS,et al.The six mammalian cell entry proteins (Mce3A-F) encoded by the mce3 operon are expressed during in vitro growth of Mycobacterium tuberculosis[J]. Scand J Immunol, 2010, 62(1): 16-24. DOI:10.1111/j.1365-3083.2005.01639.x
[56] Song H,Sandie R,Wang Y,et al.Identification of outer membrane proteins of Mycobacterium tuberculosis[J]. Tuberculosis, 2008, 88(6): 526-44. DOI:10.1016/j.tube.2008.02.004
[57] Jiang Y,Liu H,Wang X,et al.Conserved hypothetical protein Rv1977 in Mycobacterium tuberculosis strains contains sequence polymorphisms and might be involved in ongoing immune evasion[J]. Int J Clin Exp Pathol, 2015, 8(6): 6891.
[58] Takayama K, Wang C, Besra GS.Pathway to synthesis and processing of mycolic acids in Mycobacterium tuberculosis[J]. Clin Microbiol Rev,2005,18(1):81. DOI:10.1128/CMR.18.1.81-101.2005
[59] 朱琳,徐颖,孔聪,等.结核分枝杆菌新型抗原Rv3400的免疫原性研究[J].中国防痨杂志, 2016, 38(3): 201-208. DOI:10.3969/j.issn.1000-6621.2016.03.010
[60] Li W,Zhao Q,Deng W,et al.Mycobacterium tuberculosis Rv3402c enhances mycobacterial survival within macrophages and modulates the host pro-inflammatory cytokines production via NF-kappa B/ERK/p38 signaling[J]. Plos One, 2014, 9(11): e94418. DOI: 10.1371/journal.pone.0094418
[61] 李武. 结核分枝杆菌分泌性效应分子的鉴定、功能与信号传递研究[D]. 重庆:西南大学, 2015.
[62] 闫晓晓,王洪海,张舒林. RD区编码蛋白在结核病免疫诊断中的研究进展[J].现代生物医学进展, 2010, 10(20): 3935-3940.