Abstract:The aim of this study is to investigate the prevalence of staphylococcal enterotoxin genes profile of Staphylococcus aureus in pig farms samples and commercial fresh pork. Totally 18 enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser, seu) were detected in S. aureus by PCR. Of 130 S. aureus, 94.6%(123/130)isolates were positive for enterotoxin genes, including 55%(55/100)isolates from pig farms samples and 80%(24/30)isolates from commercial fresh pork. The highest positive rate of seb account for 60.8% among 18 enterotoxin genes. The main toxins gene profiles were detected in S.aureus from pig farms samples were seb+seg+sem+sen, seg+sem, seg+sei+sem+sen, seg+sem+sen and seb+seg+sei+sem+sen, and their positive rates were 8.0%(8/100), 7.0%(7/100), 7.0%(7/100)and 6.0%(6/100)respectively. The main toxins gene profiles were detected in S.aureus from commercial fresh pork were sea+seb+see, sea+seb, seb+see and sea+see+sem, and their positive rates were 30%(9/30), 10%(3/30), 10%(3/30)and 6.7%(2/30)respectively. Neither sed, sej, seo, sep, seq, ser or seu genes were detected in 130 S. aureus. S. aureus were isolated from pig farms and commercial fresh pork carrying toxin genes, and the toxins gene profiles were different, there may be different pollution sources. Our findings has certain significance for monitoring the status of S. aureus pollution and the distribution of toxin genes in pig farms and commercial fresh pork.
邹忠爱. 生猪养殖环境及猪肉中金黄色葡萄菌肠毒素基因分布研究[J]. 中国人兽共患病学报, 2018, 34(5): 441-446.
ZOU Zhong-ai. Detection of staphylococcal enterotoxin genes in Staphylococcus aureus isolates from pig farms and commercial fresh pork. Chinese Journal of Zoonoses, 2018, 34(5): 441-446.
[1] Crombe F, Argudin MA, Vanderhaeghen W, et al.Transmission dynamics of methicillin-resistant Staphylococcus aureus in pigs[J]. Front Microbiol, 2013, 4(57):1-21.DOI:10.3389/fmicb.2013.00057
[2] Kadariya J, Smith TC, Thapaliya D.Staphylococcus aureus and staphylococcal food-borne disease: an ongoing challenge in public health[J]. Biomed Res Int, 2014, 4(2014):1-9. DOI: 10.1155/2014/827965
[3] 陈彬,黄晓蓉,汤敏英,等.基因探针法快速检测食品中金黄色葡萄球菌的研究[J]. 现代预防医学杂志, 2014, 34(6): 1017-1020.
[4] Lawrynowicz-Paciorek M, Kochman M, Piekarska K, et al.The distribution of enterotoxin and enterotoxin-like genes in Staphylococcus aureus strains isolated from nasal carriers and food samples[J]. Int J Food Microbiol, 2007,117(3):319-323. DOI: 10.1016/j.ijfoodmicro.2007.03.009
[5] Wu S, Duan N, Gu H, et al.A review of the methods for detection of Staphylococcus aureus enterotoxins[J].Toxins, 2016, 8(7):176. DOI: 10.3390/toxins8070176
[6] Akineden O, Annemuller C, Hassan AA, et al.Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis[J]. Clin Diagn Lab Immunol, 2001, 8(5):959-964. DOI: 10.1128/CDLI.8.5.959-964.2001
[7] Kot B, Szweda P, Frankowska-Maciejewska A, et al.Virulence gene profiles in Staphylococcus aureus isolated from cows with subclinical mastitis in eastern Poland[J]. J Dairy Res, 2016, 83(2):228-235. DOI: 10.1017/S002202991600008X
[8] 魏志恒,于宏伟,李宁,等. 金黄色葡萄球菌溶血素基因分布的研究[J]. 中国食品学报, 2009,9(6):152-156.
[9] Nishifuji K, Sugai M, Amagai M.Staphylococcal exfoliative toxins: "molecular scissors" of bacteria that attack the cutaneous defense barrier in mammals[J]. J Dermatol Sci, 2008, 49(1):21-31. DOI: 10.1016/j.jdemsci.2007.05.007
[10] Ruzickova V, Voller J, Pantucek R, et al.Multiplex PCR for detection of three exfoliative toxin serotype genes in Staphylococcus aureus[J]. Folia Microbiol (Praha), 2005, 50(6):499-502. DOI: 10.1007/BF02931437
[11] Koop G, Vrieling M, Storisteanu DM, et al.Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus[J]. Sci Rep, 2017,1(7):1-10. DOI: 10.1038/srep40660
[12] Argudin MA, Tenhagen BA, Fetsch A, et al.Virulence and resistance determinants of German Staphylococcus aureus ST398 isolates from nonhuman sources[J]. Appl Environ Microbiol, 2011, 77(9):3052-3060. DOI;10.1128/AEM.02260-10
[13] Lovseth A, Loncarevic S, Berdal KG.Modified multiplex PCR method for detection of pyrogenic exotoxin genes in Staphylococcal isolates[J]. J Clin Microbiol, 2004, 42(8):3869-3872. DOI: 10.1128/JCM.42.8.3869-3872.2004
[14] Tristan A, Ying L, Bes M, et al.Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections[J]. J Clin Microbiol, 2003, 41(9):4465-4467. DOI: 10.1128/JCM.41.9.4465-4467.2003
[15] Peacock SJ, Moore CE, Justice A, et al.Virulent combinations of adhesin and toxin genes in natural populations of Staphylococcus aureus[J]. Infect Immun, 2002, 70(9):4987-4996. DOI: 10.1128/IAI.70.9.4987-4996.2002
[16] Moore P, Lindsay JA.Genetic variation among hospital isolates of methicillin-sensitive Staphylococcus aureus: Evidence for horizontal transfer of virulence genes[J]. J Clin Microbiol,2001, 39(8):2760-2767. DOI:10.1128/JCM.39.8.2760-2767.2001
[17] 汪永禄,张萍,陶勇,等.不同来源金黄色葡萄球菌肠毒素及耐药性检测分析[J].实用预防医学, 2014, 21(4):488-491.
[18] 沈玄艺,宋启发,徐景野,等. 食源性金黄色葡萄球菌肠毒素基因型分布研究[J].中国食品卫生杂志, 2012, 24(5):427-429.
[19] 沈伟伟,裘丹红,徐佳,等. 台州市食源性金黄色葡萄球菌的主动监测及肠毒素基因分布研究[J]. 中国卫生检验杂志, 2011, 21(11):2674-2676.
[20] 章乐怡,李毅,马雪莲.食物中毒及食品中金黄色葡萄球菌的肠毒素及基因的研究[J].中国卫生检验杂志, 2009, 19(12):2851-2853.